Antagonistic activity of probiotic lactobacilli and bifidobacteria against entero- and uropathogens
| Autor: | Krista Lõivukene, J. Shchepetova, Pirje Hütt, Marika Mikelsaar, Tiiu Kullisaar |
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| Rok vydání: | 2006 |
| Předmět: |
Bifidobacterium longum
Lactobacillus paracasei Lactobacillus fermentum Digestive System Diseases Applied Microbiology and Biotechnology Antioxidants Statistics Nonparametric Microbiology fluids and secretions Lactobacillus acidophilus Species Specificity Lactobacillus rhamnosus Lactobacillus Antibiosis Humans Bifidobacterium biology Probiotics food and beverages Bacteriology General Medicine Hydrogen-Ion Concentration Fatty Acids Volatile Urination Disorders biology.organism_classification Culture Media bacteria Lactobacillus plantarum Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 100:1324-1332 |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1111/j.1365-2672.2006.02857.x |
| Popis: | Aim: To develop in vitro assays for comparing the antagonistic properties and anti-oxidative activity of probiotic Lactobacillus and Bifidobacterium strains against various entero- and urinary pathogens. Methods and Results: The antagonistic activity of five probiotic lactobacilli (Lactobacillus rhamnosus GG, Lactobacillus fermentum ME-3, Lactobacillus acidophilus La5, Lactobacillus plantarum 299v and Lactobacillus paracasei 8700:2) and two bifidobacteria (Bifidobacterium lactis Bb12, Bifidobacterium longum 46) against six target pathogens was estimated using different assays (solid and liquid media, anaerobic and microaerobic cultivation) and ranked (low, intermediate and high). Bacterial fermentation products were determined by gas chromatography, and the total anti-oxidative activity of probiotics was measured using linolenic acid test. Pyelonephritic Escherichia coli was highly suppressed by GG and both bifidobacteria strains. Lactobacilli strains 8700:2, 299v and ME-3 were the most effective against Salmonella enterica ssp. enterica in microaerobic while ME-3 and both bifidobacteria expressed high activity against Shigella sonnei in anaerobic milieu. Lact. paracasei, Lact. rhamnosus and Lact. plantarum strains showed intermediate antagonistic activity against Helicobacter pylori under microaerobic conditions on solid media. The highest antioxidative activity was characteristic for Lact. fermentum ME-3 (P |
| Databáze: | OpenAIRE |
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