The use of a personal glucose meter for detecting procalcitonin through glucose encapsulated within liposomes
| Autor: | Richard D. Tilley, Fida’A. Alshawawreh, J. Justin Gooding, Nicholas Ariotti, Tania M. Benedetti, Fabio Lisi, Padmavathy Bakthavathsalam |
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| Rok vydání: | 2019 |
| Předmět: |
Capsules
02 engineering and technology 01 natural sciences Biochemistry Procalcitonin Analytical Chemistry Pulmonary surfactant Limit of Detection parasitic diseases Electrochemistry medicine Humans Environmental Chemistry Spectroscopy Whole blood Immunoassay Detection limit Liposome Reproducibility Chromatography Chemistry Blood Glucose Self-Monitoring Glucose meter 010401 analytical chemistry bacterial infections and mycoses 021001 nanoscience & nanotechnology Human serum albumin 0104 chemical sciences Kinetics Glucose Liposomes 0210 nano-technology hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | The Analyst. 144:6225-6230 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c9an01519a |
| Popis: | Herein, a glucose meter-based immunosensing platform is developed that allows the quantification of procalcitonin (PCT) in whole blood samples. PCT is a biomarker for sepsis and its early detection would improve the safety of the patient, as the diagnostic process will be easier and faster. The method employs liposomes with encapsulated glucose as a signal generation tag, which are then used in a sandwich immunoassay by conjugating an antibody to the liposome. The optimal liposomes' size and concentration of encapsulated glucose is determined experimentally to be 200 nm and 27.8 mM, respectively. Upon the addition of a surfactant (Triton X-100), the glucose is released and a signal is detected with a personal glucose meter (PGM). This signal is directly proportional to the concentration of the PCT in the sample. The dynamic range of the assay developed was 0.153-15.38 nM, and could allow the detection of PCT as low as 0.15 nM. The assay showed a high selectivity toward PCT against other proteins such as C-reactive protein and human serum albumin and good reproducibility. This assay was able to quantitatively determine the amount of PCT in whole blood samples at clinically-relevant concentrations. |
| Databáze: | OpenAIRE |
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