New in vitro assay based on glucose consumption for determining intraconazole and amphotericin B activities against Aspergillus fumigatus
| Autor: | J. P. Seguela, M Lagente, M. D. Linas, J C Garrigues, G. Cadet De Fontenay |
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| Rok vydání: | 1994 |
| Předmět: |
Pharmacology
biology Itraconazole Aspergillus fumigatus Microbial Sensitivity Tests Fungi imperfecti Carbohydrate metabolism biology.organism_classification In vitro Microbiology Incubation period Glucose Infectious Diseases Amphotericin B Carbon source medicine Pharmacology (medical) Research Article medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 38:2857-2862 |
| ISSN: | 1098-6596 0066-4804 |
| Popis: | We developed a new in vitro method of evaluating antifungal molecules. Fungal growth was determined by measuring glucose consumption, the only carbon source in a synthetic medium. First, the growth of 12 Aspergillus fumigatus strains was studied. Glucose consumption was an excellent indicator of fungal growth. Second, the partial inhibition of growth was calculated in terms of the 90% or 50% inhibitory concentration for the 12 strains after treatment with itraconazole and amphotericin B. With a 3-day incubation time, the calculated 90% and 50% inhibitory concentrations agreed with those obtained by a macromethod and with those reported in previous publications. In each case the high degrees of efficacy of itraconazole and amphotericin B against A. fumigatus were confirmed. Partial inhibition induced by low concentrations of antifungal agents was quantifiable by this new method. |
| Databáze: | OpenAIRE |
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