Long term stability and infectivity of herpesviruses in water
| Autor: | Armando Damiani, Anisha Dayaram, Alexander Schattschneider, Mathias Franz, Nikolaus Osterrieder, Sebastian Bischofberger, Alex D. Greenwood |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Viral vectors Time Factors 040301 veterinary sciences media_common.quotation_subject Otras Ciencias Biológicas 030106 microbiology Zoology Biology Article Cell Line 0403 veterinary science Ciencias Biológicas purl.org/becyt/ford/1 [https] 03 medical and health sciences Viral reservoirs EHV-1 Animals Herpes virus Horses Turbidity purl.org/becyt/ford/1.6 [https] media_common Infectivity Multidisciplinary Microbial Viability Longevity Water 04 agricultural and veterinary sciences Herpesviridae Infections stability Virology Salinity Sympatric speciation Vector (epidemiology) Soil water Rabbits Equine herpesvirus Water Microbiology CIENCIAS NATURALES Y EXACTAS Herpesvirus 1 Equid |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Scientific reports, 7:46559 Scientific Reports |
| Popis: | For viruses to utilize environmental vectors (hard surfaces, soil, water) for transmission, physical and chemical stability is a prerequisite. There are many factors including pH, salinity, temperature, and turbidity that are known to contribute to the ability of viruses to persist in water. Equine herpesvirus type-1 (EHV-1) is a pathogenic alphaherpesvirus associated with domestic horses and wild equids. EHV-1 and recombinants of EHV-1 and EHV-9 are able to cause infections in non-equid animal species, particularly in captive settings. Many of the captive non-equid mammals are not naturally sympatric with equids and do not share enclosures, however, in many cases water sources may overlap. Similarly, in the wild, equids encounter many species at waterholes in times of seasonal drought. Therefore, we hypothesized that EHV-1 is stable in water and that water may act as a vector for EHV-1. In order to establish the conditions promoting or hindering EHV-1 longevity, infectivity and genomic stability in water; we exposed EHV-1 to varied water environments (pH, salinity, temperature, and turbidity) in controlled experiments over 21 days. The presence and infectivity of the virus was confirmed by both qPCR and cell culture experiments. Our results show that EHV-1 remains stable and infectious under many conditions in water for up to three weeks. Fil: Dayaram, Anisha. Leibniz Institute For Zoo And Wildlife Research; Alemania Fil: Franz, Mathias. Leibniz-institute For Zoo And Wildlife Research; Alemania Fil: Schattschneider, Alexander. Leibniz-institute For Zoo And Wildlife Research; Alemania Fil: Damiani, Armando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Freie University Berlin; Alemania Fil: Bischofberger, Sebastian. Freie University Berlin; Alemania Fil: Osterrieder, Nikolaus. Freie University Berlin; Alemania Fil: Greenwood, Alex D.. Leibniz-institute For Zoo And Wildlife Research; Alemania |
| Databáze: | OpenAIRE |
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