Pseudomonas tarimensis sp. nov., an endophytic bacteria isolated from Populus euphratica
| Autor: | Bolatkhan K. Zayadan, Nusratgul Anwar, Erkin Rahman, Marygul Kurban, Mehfuzem Abdurahman, Tursunay Mamtimin, Manziram Rozahon, Hormathan Mamtimin, Muhtar Abdukerim, Mihribangul Abdurusul |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial China Ubiquinone medicine.disease_cause Microbiology 03 medical and health sciences Pseudomonas indica Azotobacter salinestris Pseudomonas RNA Ribosomal 16S Botany medicine Pseudomonas azotifigens Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Base Composition biology Strain (chemistry) Plant Stems Pseudomonas balearica Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA rpoB Bacterial Typing Techniques 030104 developmental biology Populus |
| Zdroj: | International journal of systematic and evolutionary microbiology. 67(11) |
| ISSN: | 1466-5034 |
| Popis: | An endophytic bacterium, MA-69T, was isolated from the storage liquid in the stems of Populuseuphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain MA-69T was found to be short rod-shaped, Gram-stain-negative, non-spore-forming, aerobic and motile by means of a monopolar flagellum. According to phylogenetic analysis based on 16S rRNA gene sequences, strain MA-69T was assigned to the genus Pseudomonas with highest 16S rRNA gene sequence similarity of 97.5 % to Pseudomonas azotifigens JCM 12708T, followed by Pseudomonas matsuisoli JCM 30078T (97.5 %), Pseudomonas balearica DSM 6083T (97.1 %), Azotobacter salinestris ATCC 49674T (96.1 %) and Pseudomonas indica DSM 14015T (95.9 %). Analysis of strain MA-69T based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the isolate to be distinctly delineated from species of the genus Pseudomonas . The DNA G+C content of strain MA-69T was 64.1 mol%. DNA–DNA hybridization with Pseudomonas azotifigens JCM 12708T, Pseudomonas matsuisoli JCM 30078T and Pseudomonas balearica DSM 6083T revealed 62.9, 60.1 and 49.0 % relatedness, respectively. The major fatty acids in strain MA-69T were summed feature 3 (25.7 %), summed feature 8 (24.0 %), C19 : 0cyclo ω8c (19.9 %), C16 : 0 (14.6 %) and C12 : 0 (6.3 %). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Q-9 was the major quinone in strain MA-69T. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain MA-69T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas tarimensis sp. nov. is proposed. The type strain is MA-69T (=CCTCC AB 2013065T=KCTC 42447T). |
| Databáze: | OpenAIRE |
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