The generation of nitric oxide participates in gamma IFN-induced MHC class II antigen expression by cultured astrocytoma cells
| Autor: | Vincenzo Mollace, Renato Massoud, Giuseppe Nisticò, Giuliana M. Lauro, Marco Colasanti, E. Cundari |
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| Rok vydání: | 1993 |
| Předmět: |
Platelet Aggregation
medicine.medical_treatment Immunology Antigen presentation Biology Astrocytoma Arginine Nitric Oxide MHC class II antigen Interferon-gamma Antigen medicine Tumor Cells Cultured Humans Interferon gamma Antigen-presenting cell neoplasms Pharmacology Histocompatibility Antigens Class II Molecular biology In vitro nervous system diseases Methylene Blue Cytokine medicine.anatomical_structure NG-Nitroarginine Methyl Ester Biochemistry Amino Acid Oxidoreductases Nitric Oxide Synthase medicine.drug Astrocyte |
| Zdroj: | International journal of immunopharmacology. 15(6) |
| ISSN: | 0192-0561 |
| Popis: | The effects of interferon gamma (gamma IFN) on the MHCII antigen expression by human cultured astrocytoma cells were investigated. The co-incubation of gamma IFN with T67 astrocytoma cells produced a dose-dependent increase of MHCII antigen expression as evaluated by flow cytometric (FACS) analysis and confocal laser microscopy analysis. The number of MHCII molecules expressed by gamma IFN-pretreated astrocytoma cells was reduced by co-incubation with N omega-nitro-L-arginine methyl ester (L-NAME), a selective inhibitor of the nitric oxide (NO)-synthesizing enzyme. In addition, methylene blue, which inhibits the biological activity of NO acting at the guanylate cyclase level, strongly antagonized the MHCII antigen expression on astrocytoma cells induced by gamma IFN. Furthermore, gamma IFN increased the activity of the inducible isoform of NO-synthase as well as the concentration of nitrite, one of the breakdown products of NO and the antiplatelet activity of astrocytoma cells. In conclusion, the present data show that gamma IFN increases the synthesis and release of NO by cultured astrocytoma cells and this could co-participate in the MHCII antigen expression by this cell type. Therefore, the generation of NO by cultured astrocytoma cells may represent an important step in the development of the immunocompetent activity of astrocytes. |
| Databáze: | OpenAIRE |
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