Phenotypic and functional changes of T cell subsets after CoronaVac vaccination
| Autor: | Wisitsak Phoksawat, Arnone Nithichanon, Hatairat Lerdsamran, Surasakdi Wongratanacheewin, Atibordee Meesing, Chonlatip Pipattanaboon, Sakawrat Kanthawong, Sirinart Aromseree, Umaporn Yordpratum, Marut Laohaviroj, Viraphong Lulitanond, Sorujsiri Chareonsudjai, Pilaipan Puthavathana, Ludthawun Kamuthachad, Chatcharin Kamsom, Chakrit Thapphan, Kanin Salao, Arunya Chonlapan, Punnapat Nawawishkarun, Jarunee Prasertsopon, Hans J. Overgaard, Steven W. Edwards, Supranee Phanthanawiboon |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
COVID-19 Vaccines
General Veterinary General Immunology and Microbiology SARS-CoV-2 Vaccination Public Health Environmental and Occupational Health COVID-19 Viral Vaccines Th1 Cells Antibodies Viral Antibodies Neutralizing Infectious Diseases Vaccines Inactivated Immunoglobulin G Molecular Medicine Humans |
| Zdroj: | Vaccine. 40(48) |
| ISSN: | 1873-2518 |
| Popis: | The pandemic coronavirus disease 2019 (COVID-19) is a major global public health concern and several protective vaccines, or preventive/therapeutic approaches have been developed. Sinovac-CoronaVac, an inactivated whole virus vaccine, can protect against severe COVID-19 disease and hospitalization, but less is known whether it elicits long-term T cell responses and provides prolonged protection.This is a longitudinal surveillance study of SARS-CoV-2 receptor binding domain (RBD)-specific IgG levels, neutralizing antibody levels (NAb), T cell subsets and activation, and memory B cells of 335 participants who received two doses of CoronaVac. SARS-CoV-2 RBD-specific IgG levels were measured by enzyme-linked immunosorbent assay (ELISA), while NAb were measured against two strains of SARS-CoV-2, the Wuhan and Delta variants. Activated T cells and subsets were identified by flow cytometry. Memory B and T cells were evaluated by enzyme-linked immune absorbent spot (ELISpot).Two doses of CoronaVac elicited serum anti-RBD antibody response, elevated B cells with NAb capacity and CD4CoronaVac mainly activated B cells but T cells, especially Th1 cells, were poorly activated. Activated T cells were mainly Th2 biased, demonstrating development of effector B cells but not long-lasting memory plasma cells. Taken together, these results suggest that protection with CoronaVac is short-lived and that a third booster dose of vaccine may improve protection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect