A multicentre analysis of four low-density lipoprotein cholesterol direct assays in samples with extreme high-density lipoprotein cholesterol concentrations
| Autor: | María Pilar Loeches-Jiménez, Teodoro J. Palomino-Muñoz, Luis Sáenz-Mateos, Manuel Gómez-Serranillos, Ana Arpa-Fernández, Emilio J Laserna-Mendieta, Jesús Timón-Zapata, Lucía Ruiz-Trujillo |
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| Rok vydání: | 2013 |
| Předmět: |
Chromatography
Models Statistical Cholesterol Biochemistry (medical) Clinical Biochemistry Cholesterol HDL Analytical chemistry Low density lipoprotein cholesterol General Medicine Cholesterol LDL Biochemistry Risk category chemistry.chemical_compound High-density lipoprotein chemistry Homogeneous Total cholesterol Disease risk Humans lipids (amino acids peptides and proteins) Poor correlation |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 430 |
| ISSN: | 1873-3492 |
| Popis: | Background Although LDL-C has been traditionally estimated using the Friedewald formula (FF), several direct homogeneous assays have been developed to overcome the limitations of this formula and the complicated manual procedure required in the reference method. However, several differences have been reported between these assays in certain situations. Methods Two groups of 105 samples with extreme low and high HDL-C concentrations were processed, employing four different instruments and with the reagents for total cholesterol, triglycerides, HDL-C and LDL-C provided by the distinct manufacturers. Results Statistical tests indicated important differences between HDL-C and LDL-C homogeneous methods. Poor correlation, significant bias and high discrepancy in cardiovascular disease risk classification were observed for LDL-C direct assays in the low HDL-C group, whereas better results were obtained when comparing LDL-C levels estimated with the FF. In contrast, three of the four instruments generated LDL-C direct results with a good agreement in the high HDL-C group, even though an appreciable misclassification percentage in risk categories must be taken into account. Conclusions Our results indicate that extreme low or high HDL-C levels can represent a non-previously described source of variation between commercially available LDL-C homogeneous assays. |
| Databáze: | OpenAIRE |
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