Protein Corona Inhibits Endosomal Escape of Functionalized DNA Nanostructures in Living Cells
| Autor: | Minghui Liu, Skylar J. W. Henry, Nicholas Stephanopoulos, Martin Hof, Piotr Jurkiewicz, Mariia Lunova, Barbora Smolková, Mariia Uzhytchak, Alexandr Dejneka, Tara MacCulloch, Tyler F Rockwood, Adam Frtús, Oleg Lunov |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Materials science Endosome Vesicle fungi Protein Corona Peptide Biological activity DNA Endosomes Article Nanostructures Dna nanostructures chemistry Cell Line Tumor DNA nanotechnology Biophysics Hepatic stellate cell Humans Nucleic Acid Conformation General Materials Science Adsorption Lysosomes Antimicrobial Cationic Peptides |
| Zdroj: | ACS Appl Mater Interfaces |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.1c14401 |
| Popis: | DNA nanostructures (DNs) can be designed in a controlled and programmable manner, and these structures are increasingly used in a variety of biomedical applications, such as the delivery of therapeutic agents. When exposed to biological liquids, most nanomaterials become covered by a protein corona, which in turn modulates their cellular uptake and the biological response they elicit. However, the interplay between living cells and designed DNs are still not well established. Namely, there are very limited studies that assess protein corona impact on DN biological activity. Here, we analyzed the uptake of functionalized DNs in three distinct hepatic cell lines. Our analysis indicates that cellular uptake is linearly dependent on the cell size. Further, we show that the protein corona determines the endolysosomal vesicle escape efficiency of DNs coated with an endosome escape peptide. Our study offers an important basis for future optimization of DNs as delivery systems for various biomedical applications. |
| Databáze: | OpenAIRE |
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