Specific detection of Histomonas meleagridis in turkeys by a PCR assay with an internal amplification control
| Autor: | Jeroen Koen De Gussem, Bruno Goddeeris, Nele Bleyen, Koen De Gussem |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Turkeys
Specific detection Pcr assay Polymerase Chain Reaction Sensitivity and Specificity Histomonas meleagridis Histomoniasis medicine Animals False Negative Reactions Protozoan Infections Animal Poultry Diseases DNA Primers General Veterinary biology Base Sequence Gene Amplification Eukaryota Blackhead disease General Medicine DNA Protozoan medicine.disease biology.organism_classification Virology Specific primers Parasitology |
| Zdroj: | Veterinary parasitology. 143(3-4) |
| ISSN: | 0304-4017 |
| Popis: | Histomoniasis or blackhead is a disease of gallinaceous birds, caused by the protozoon Histomonas meleagridis. Since traditional diagnostics for the detection of this disease are complex and far less sensitive than molecular tools, a PCR would provide a more rapid and sensitive alternative. However, intestinal material and droppings, which are preferably used in epidemiological studies of histomoniasis, often contain PCR inhibitory substances. To detect these false negative results, the use of an internal amplification control is essential. Nevertheless, the recently developed PCR tests lack this internal control. Therefore, a new PCR assay with H. meleagridis specific primers was developed which does include an internal amplification control. The diagnostic value of the PCR assay was evaluated in comparison to three other conventional H. meleagridis specific PCR tests (HIS5, HM1 and HM2). None of the organ samples originating from uninfected turkeys, showed positive PCR results in any of the tests. Among the lesion-positive, inhibition-free samples, 95.4% were positive by our PCR assay, while only 50, 66.7 and 83.3% of the lesion-positive organs tested positive by the HM1, the HIS5 and the HM2 PCR respectively. In conclusion, our PCR offers the use of the internal control to detect false negative results and an increased sensitivity, and thus should be useful for routine diagnosis of H. meleagridis in poultry. |
| Databáze: | OpenAIRE |
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