Assembly and Characterization of HBc Derived Virus-like Particles with Magnetic Core
| Autor: | Marcin Pyrski, Jakub Dalibor Rybka, Alicja Plis, Tomasz Pniewski, Michael Giersig, Adam Aron Mieloch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Antigenicity
HBcAg General Chemical Engineering viruses Nanoparticle Nicotiana benthamiana Ionic bonding 02 engineering and technology virus-like particles complex mixtures Article Virus magnetic core lcsh:Chemistry 03 medical and health sciences hepatitis B virus capsid protein VLPs General Materials Science 030304 developmental biology 0303 health sciences viral self-assembly biology Chemistry HBc virus diseases 021001 nanoscience & nanotechnology biology.organism_classification Capsid lcsh:QD1-999 Transmission electron microscopy Biophysics 0210 nano-technology |
| Zdroj: | Nanomaterials, Vol 9, Iss 2, p 155 (2019) Nanomaterials Volume 9 Issue 2 |
| ISSN: | 2079-4991 |
| Popis: | Core-virus like particles (VLPs) assembly is a kinetically complex cascade of interactions between viral proteins, nanoparticle&rsquo s surface and an ionic environment. Despite many in silico simulations regarding this process, there is still a lack of experimental data. The main goal of this study was to investigate the capsid protein of hepatitis B virus (HBc) assembly into virus-like particles with superparamagnetic iron oxide nanoparticles (SPIONs) as a magnetic core in relation to their characteristics. The native form of HBc was obtained via agroinfection of Nicotiana benthamiana with pEAQ-HBc plasmid. SPIONs of diameter of 15 nm were synthesized and functionalized with two ligands, providing variety in &zeta potential and hydrodynamic diameter. The antigenic potential of the assembled core-VLPs was assessed with enzyme-linked immunosorbent assay (ELISA). Morphology of SPIONs and core-VLPs was evaluated via transmission electron microscopy (TEM). The most successful core-VLPs assembly was obtained for SPIONs functionalized with dihexadecyl phosphate (DHP) at SPIONs/HBc ratio of 0.2/0.05 mg/mL. ELISA results indicate significant decrease of antigenicity concomitant with core-VLPs assembly. In summary, this study provides an experimental assessment of the crucial parameters guiding SPION-HBc VLPs assembly and evaluates the antigenicity of the obtained structures. |
| Databáze: | OpenAIRE |
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