Effect of sperm DNA fragmentation on clinical outcomes for Chinese couples undergoing in vitro fertilization or intracytoplasmic sperm injection

Autor: Yue-Yue Huang, Jun-Ping Cheng, Li Huang, Lintao Xue, Rui-Xue Wang, Wei-Ying Mo, Bing He, Ruizhi Liu, Shikai Wang, Xian-Bao Mao
Rok vydání: 2016
Předmět:
Adult
Male
fertilization rate
Pregnancy Rate
medicine.medical_treatment
intracytoplasmic sperm injection
DNA Fragmentation
Fertilization in Vitro
Biochemistry
Intracytoplasmic sperm injection
in vitro fertilization
Andrology
03 medical and health sciences
0302 clinical medicine
Human fertilization
Pregnancy
medicine
Humans
Sperm Injections
Intracytoplasmic
Infertility
Male
reproductive and urinary physiology
Retrospective Studies
030219 obstetrics & reproductive medicine
In vitro fertilisation
urogenital system
business.industry
Biochemistry (medical)
Embryogenesis
Pregnancy Outcome
Research Reports
Oligospermia
Cell Biology
General Medicine
Embryo Transfer
medicine.disease
Spermatozoa
Sperm
Sperm DNA fragmentation
ROC Curve
030220 oncology & carcinogenesis
embryonic structures
Oocytes
Sperm Motility
DNA fragmentation
Female
business
therapeutics
Zdroj: The Journal of International Medical Research
ISSN: 1473-2300
0300-0605
DOI: 10.1177/0300060516664240
Popis: Objective To investigate the effect of sperm DNA fragmentation on the fertilization rate, embryo development and pregnancy outcome of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a cohort of Chinese couples. Methods Infertile couples that had undergone assisted reproductive technology at our centre between January 2011 and December 2013 were included in this retrospective study. Fractions of prepared sperm samples were evaluated for sperm DNA fragmentation on the day of oocyte recovery. Results Of the 550 couples selected, 415 had undergone IVF and 135 ICSI. Sperm DNA fragmentation rate was significantly negatively correlated with the fertilization rate in the ICSI cycles but not the IVF cycles. No association was found between sperm DNA fragmentation and cleavage rate or good quality embryo formation rates in IVF or ICSI cycles. Receiver operating characteristic (ROC) curve analysis showed that the sperm DNA fragmentation rate was a statistically significant prognostic indicator of the clinical fertilization rate in ICSI cycles; a rate > 22.3% was associated with a lower fertilization rate following ICSI compared with a rate ≤ 22.3%. Conclusions High values of sperm DNA fragmentation were associated with a low fertilization rate following ICSI but were not associated with alterations in pregnancy or live birth rates in either ICSI or IVF in this cohort of Chinese couples.
Databáze: OpenAIRE
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