Near-infrared labeled, ovalbumin loaded polymeric nanoparticles based on a hydrophilic polyester as model vaccine: In vivo tracking and evaluation of antigen-specific CD8+ T cell immune response
| Autor: | Rahimian, Sima, Kleinovink, Jan Willem, Fransen, Marieke F., Mezzanotte, Laura, Gold, Henrik, Wisse, Patrick, Overkleeft, Hermen, Amidi, Maryam, Jiskoot, Wim, Lo¨wik, Clemens W., Ossendorp, Ferry, Hennink, Wim E., Sub Drug delivery, UIPS - Utrecht Institute for Pharmaceutical Sciences, Pharmaceutics |
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| Přispěvatelé: | Sub Drug delivery, UIPS - Utrecht Institute for Pharmaceutical Sciences, Pharmaceutics |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Infrared Rays Ovalbumin Polyesters medicine.medical_treatment Antigen delivery Biophysics Bioengineering CD8-Positive T-Lymphocytes PLHMGA Biomaterials Epitopes Immune system Antigen In vivo medicine Animals Cytotoxic T cell Antigen Presentation Vaccines In vivo tracking Cell Death Staining and Labeling biology Immunity Dendritic Cells Immunotherapy In vitro Mice Inbred C57BL Near-infrared imaging Mechanics of Materials Immunology biology.protein Ceramics and Composites Nanoparticles Hydrophobic and Hydrophilic Interactions CD8 |
| Zdroj: | Biomaterials, 37, 469. Elsevier Ltd Biomaterials, 37, 469-477 |
| ISSN: | 0142-9612 |
| Popis: | Particulate antigen delivery systems aimed at the induction of antigen-specific T cells form a promising approach in immunotherapy to replace pharmacokinetically unfavorable soluble antigen formulations. In this study, we developed a delivery system using the model protein antigen ovalbumin (OVA) encapsulated in nanoparticles based on the hydrophilic polyester poly(lactide-co-hydroxymethylglycolic acid) (pLHMGA). Spherical nanoparticles with size 300-400 nm were prepared and characterized and showed a strong ability to deliver antigen to dendritic cells for cross-presentation to antigen-specific T cells in vitro. Using near-infrared (NIR) fluorescent dyes covalently linked to both the nanoparticle and the encapsulated OVA antigen, we tracked the fate of this formulation in mice. We observed that the antigen and the nanoparticles are efficiently co-transported from the injection site to the draining lymph nodes, in a more gradual and durable manner than soluble OVA protein. OVA-loaded pLHMGA nanoparticles efficiently induced antigen cross-presentation to OVA-specific CD8+ T cells in the lymph nodes, superior to soluble OVA vaccination. Together, these data show the potential of pLHMGA nanoparticles as attractive antigen delivery vehicles. |
| Databáze: | OpenAIRE |
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