Detection of genetic variability in various isolates of cattle tick, Boophilus microplus from Tamil Nadu, India using PCR-RAPD analysis
| Autor: | Sakthivelkumar Shanmugavel, Veeramani Velayutham, Janarthanan Sundaram, Arumugam Munusamy |
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| Rok vydání: | 2012 |
| Předmět: |
Veterinary medicine
India Tick Polymorphism (computer science) parasitic diseases Genotype Rhipicephalus Animals Cluster Analysis Genetic variability Phylogeny Polymorphism Genetic Base Sequence Ecology biology business.industry fungi Dendrogram Genetic Variation Sequence Analysis DNA General Medicine bacterial infections and mycoses biology.organism_classification Random Amplified Polymorphic DNA Technique Biotechnology RAPD genomic DNA Animal ecology Insect Science Female business |
| Zdroj: | Experimental and Applied Acarology. 56:375-383 |
| ISSN: | 1572-9702 0168-8162 |
| DOI: | 10.1007/s10493-012-9524-2 |
| Popis: | The genomic DNA from ten isolates of the cattle tick, Boophilus microplus collected in and around Chennai, India, was analyzed by random amplified polymorphic DNA (RAPD) using PCR. Selected five random primers were used for the study of genetic variability among different isolates of B. microplus. A high degree of genetic polymorphism with a different pattern of RAPD profiles for each tick isolate was detected with all these random primers. This variability was also confirmed by similarity coefficient values and dendrogram which were performed using mean RAPD profiles for all the primers between various isolates of ticks. The findings suggest the existence of a complex genotypic diversity of the tick B. microplus in an endemic region such as Chennai. |
| Databáze: | OpenAIRE |
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