Novel components of human mitotic chromosomes identified by proteomic analysis of the chromosome scaffold fraction
| Autor: | Alexander J. Henzing, William C. Earnshaw, Reto Gassmann |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Proteomics
Recombinant Fusion Proteins Cell Cycle Centromere Green Fluorescent Proteins Mitosis Nuclear Proteins Cell cycle Biology Mass Spectrometry Cell biology Green fluorescent protein Non-histone protein Heterochromatin Genetics Chromosomes Human Humans Nuclear protein Genetics (clinical) Function (biology) |
| Zdroj: | Chromosoma. 113:385-397 |
| ISSN: | 1432-0886 0009-5915 |
| DOI: | 10.1007/s00412-004-0326-0 |
| Popis: | Chromosomal nonhistone proteins have important roles in mitotic chromosome formation and dynamics. In order to identify novel abundant proteins with a potential involvement in these processes, we initiated a proteomic screen of the chromosome scaffold fraction. This screen identified 79 proteins, 30 of which had not previously been described as components of mitotic chromosomes. Furthermore, half of these proteins had no documented function. We analyzed the cell-cycle dependent distribution of three uncharacterized proteins by expressing them as green fluorescent protein (GFP) fusions and showed that they associate with mitotic chromosomes in vivo. One of the proteins, nuclear protein p30, is a novel component of the inner centromere. Over-expression experiments indicated that p30 may have an active role in the formation of centromeric heterochromatin. |
| Databáze: | OpenAIRE |
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