Protection provided by a recombinant ALVAC(®)-WNV vaccine expressing the prM/E genes of a lineage 1 strain of WNV against a virulent challenge with a lineage 2 strain
| Autor: | Tamás Bakonyi, L. Cupillard, Richard A. Bowen, B. Powers, Jules Maarten Minke, S. Boyum, Leonardo Siger, Norbert Nowotny |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Lineage (genetic) animal diseases viruses Cross Protection Virulence Antibodies Viral Viral Envelope Proteins biology.animal medicine Animals Horses West Nile Virus Vaccines Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Vaccination Public Health Environmental and Occupational Health virus diseases Horse Viral Vaccines medicine.disease Virology nervous system diseases Titer Infectious Diseases Treatment Outcome Immunology biology.protein Molecular Medicine Female Horse Diseases Equidae Antibody West Nile virus Encephalitis West Nile Fever |
| Zdroj: | Vaccine. 29(28) |
| ISSN: | 1873-2518 |
| Popis: | The emergence of lineage 2 strains of WNV in Europe as a cause of clinical disease and mortality in horses raised the question whether the existing WNV vaccines, all based on lineage 1 strains, protect against circulating lineage 2 strains of WNV. In the present paper we have determined the level of cross protection provided by the recombinant ALVAC(®)-WNV vaccine in a severe challenge model that produces clinical signs of WNV type 2 disease. Ten horses were vaccinated twice at 4 weeks interval with one dose of the ALVAC-WNV vaccine formulated at the minimum protective dose. A further 10 horses served as controls. Two weeks after the second vaccination, all horses were challenged intrathecally with a recent neurovirulent lineage 2 strain of WNV. The challenge produced viraemia in 10 out of 10 and encephalitis in 9 out of 10 control horses. Three horses had to be euthanized for humane reasons. In contrast, none of the vaccinated horses developed WNV disease and only 1 vaccinated horse became viraemic at a single time point at low titre. The prevalence of WNV disease and viraemia were significantly lower in the vaccinated horses than in the control horses (P |
| Databáze: | OpenAIRE |
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