A Sensitive Fluorometric Method for Measurement of Vascular Permeability in Spinal Cord Injury
| Autor: | Zhi-Xiang Qu, Edward L. Hogan, Jian Xu, Phanor L. Perot |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pathology
medicine.medical_specialty Vascular permeability Capillary Permeability Necrosis chemistry.chemical_compound Animals Medicine Fluorescein Spinal cord injury Spinal Cord Injuries business.industry Dextrans Rats Inbred Strains Hydrogen-Ion Concentration Fluoresceins medicine.disease Spinal cord Extravasation Rats Dextran medicine.anatomical_structure Spinal Cord chemistry Permeability (electromagnetism) Anesthesia Female Stress Mechanical Neurology (clinical) business Fluorescein-5-isothiocyanate Blood vessel |
| Zdroj: | Journal of Neurotrauma. 8:149-156 |
| ISSN: | 1557-9042 0897-7151 |
| DOI: | 10.1089/neu.1991.8.149 |
| Popis: | A sensitive fluorometric method was modified for the evaluation of drug action upon vascular permeability in rat spinal cord injury. Fluorescein isothiocyanate-conjugated dextran (FITC-D MW 71,200), used as a macromolecular tracer, was injected iv 2 hours before sacrifice. The optimal pH for FITC-D fluorescence was 8.2. The recovery in spinal cord was 101.4 ± 4.0% (mean ± SD). The extent of FITC-D extravasation, expressed as the vascular injury index (VII), was increased in proportion to the trauma force. The peak of VII after trauma was at 2 hours. This fluorometric method is sensitive, simple, and reliable for evaluation of drug effects upon vascular permeability in CNS trauma. |
| Databáze: | OpenAIRE |
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