Antitumor Activity of Diterpenoids from Jatropha gossypiifolia: Cell Cycle Arrest and Apoptosis-Inducing Activity in RKO Colon Cancer Cells
Autor: | Zhi-Cheng Lu, Chun-Yan Zhang, Khalid Rahman, Li-Jun Zhang, Jian-Yong Zhu, Chen-Yun Ma, Ying Ye, Hong Zhang |
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Rok vydání: | 2018 |
Předmět: |
RM
Cell cycle checkpoint Colorectal cancer Pharmaceutical Science Apoptosis Jatropha 010402 general chemistry 01 natural sciences Analytical Chemistry Jatropha gossypiifolia X-Ray Diffraction Cell Line Tumor Drug Discovery medicine Humans Cytotoxicity IC50 Pharmacology Dose-Response Relationship Drug Molecular Structure Plant Stems biology Plant Extracts Terpenes 010405 organic chemistry Chemistry Circular Dichroism Organic Chemistry Cell Cycle Checkpoints medicine.disease biology.organism_classification Antineoplastic Agents Phytogenic Molecular biology Terpenoid 0104 chemical sciences Plant Leaves Complementary and alternative medicine Cell culture Molecular Medicine Diterpenes |
Zdroj: | Journal of Natural Products. 81:1701-1710 |
ISSN: | 1520-6025 0163-3864 |
Popis: | Nine new minor diterpenoids, jatrogossones A-I (1-9), and six known analogues (10-15) were separated from an extract of the branches and leaves of Jatropha gosspiifolia. Compounds 4-6 and 10, possessing a 5/11 fused-ring skeleton, and 8, 9, and 13, with a 5/9/5 fused-ring skeleton, represent rare diterpenoid skeletons that have been found only in compounds isolated from plants of the Jatropha genus. The absolute configurations of 1-10 were defined by using a combination of electronic circular dichroism data analysis and single-crystal X-ray diffraction data. The cytotoxicity of the diterpenoids was evaluated using RKO and LOVO colon cancer cells in which regenerating islet-derived protein 3-alpha (Reg3A) is highly expressed. Compound 12 exhibited cytotoxicity against RKO colon cancer cells with an IC50 value of 2.6 μM. Morphological features of apoptosis and antimigration activities were evaluated in 12-treated RKO cells. Compound 12 effectively induced apoptosis of RKO, which was associated with G2/M-phase cell cycle arrest. Flow cytometric analysis showed that the treatment by 12 significantly induced RKO cell apoptosis in a dose-dependent manner. |
Databáze: | OpenAIRE |
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