A combined approach identifies a limited number of new thyroid hormone target genes in post-natal mouse cerebellum
| Autor: | Robert L. Walker, Frédéric Flamant, Sylvie Mader, Carmen Grijota-Martínez, Romain Guyot, Paul S. Meltzer, Nathalie Allioli, Emmanuel Compe, Laure Quignodon, Jacques Samarut, David Laperrière |
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| Přispěvatelé: | Laboratoire de Biologie Moléculaire de la Cellule (LBMC), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Aging
Cerebellum MESH: Neurons MESH: Triiodothyronine Genome MESH: Mice Knockout MESH: Animals Newborn Mice 0302 clinical medicine Endocrinology MESH: Reverse Transcriptase Polymerase Chain Reaction Gene expression MESH: Gene Expression Regulation Developmental Paired Box Transcription Factors MESH: Aging MESH: Animals Oligonucleotide Array Sequence Analysis Mice Knockout Neurons Genetics 0303 health sciences Receptors Thyroid Hormone Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Developmental Cell biology medicine.anatomical_structure Triiodothyronine Genetically modified mouse Mice Inbred Strains Biology MESH: Mice Inbred Strains PAX8 Transcription Factor 03 medical and health sciences MESH: RNA MESH: Mice Inbred C57BL medicine Animals MESH: Paired Box Transcription Factors MESH: Receptors Thyroid Hormone Molecular Biology Gene MESH: Mice 030304 developmental biology Microarray analysis techniques [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology MESH: Thyroxine MESH: Cerebellum Mice Inbred C57BL Thyroxine Glial cell differentiation Animals Newborn nervous system MESH: Oligonucleotide Array Sequence Analysis RNA PAX8 030217 neurology & neurosurgery |
| Zdroj: | Journal of Molecular Endocrinology Journal of Molecular Endocrinology, BioScientifica, 2007, 39 (1), pp.17-28. ⟨10.1677/JME-06-0054⟩ Digital.CSIC. Repositorio Institucional del CSIC instname Journal of Molecular Endocrinology, 2007, 39 (1), pp.17-28. ⟨10.1677/JME-06-0054⟩ |
| ISSN: | 0952-5041 1479-6813 |
| DOI: | 10.1677/JME-06-0054⟩ |
| Popis: | Thyroid hormones act directly on gene transcription in the post-natal developing cerebellum, controlling neuronal, and glial cell differentiation. We have combined three experimental approaches to identify the target genes that are underlying this phenomenon: 1) a microarray analysis of gene expression to identify hormone responsive genes in the cerebellum of Pax8-/- mice, a transgenic mouse model of congenital hypothyroidism; 2) a similar microarray analysis on primary culture of cerebellum neurons; and 3) a bioinformatics screen of conserved putative-binding sites in the mouse genome. This identifies surprisingly a small set of target genes, which, for some of them, might be key regulators of cerebellum development and neuronal differentiation. © 2007 Society for Endocrinology. This work was supported by French Ministery of Research (ACI Biologie Cellulaire, Moléculaire et Structurale) Ligue contre le Cancer (équipe labellisée) the CASCADE European Network of Excellence (EU contract no. FOOD-CT-2004-506319) the CRESCENDO EU integrated project. |
| Databáze: | OpenAIRE |
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