Next-generation sequencing as a tool to detect vaginal microbiota disturbances during pregnancy
| Autor: | Agnieszka H. Ludwig-Slomczynska, Agnieszka Sroka-Oleksiak, Monika Brzychczy-Włoch, Wojciech Pabian, Artur Gurgul, Przemysław Kapusta, Paweł Wołkow, Tomasz Gosiewski |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Gardnerella spp Streptococcus agalactiae Atopobium vaginae GBS Lactobacillus gasseri medicine.disease_cause Microbiology Enterococcus faecalis Article 03 medical and health sciences 0302 clinical medicine Gardnerella Virology medicine Lactobacillus iners lcsh:QH301-705.5 vaginal microbiota Pregnancy biology Lactobacillus jensenii Lactobacillus spp food and beverages biology.organism_classification medicine.disease 030104 developmental biology lcsh:Biology (General) next-generation sequencing 030217 neurology & neurosurgery pregnant women |
| Zdroj: | Microorganisms Microorganisms, Vol 8, Iss 1813, p 1813 (2020) Volume 8 Issue 11 |
| Popis: | The physiological microbiota of the vagina is responsible for providing a protective barrier, but Some factors can disturb the balance in its composition. At that time, the amounts of the genus Lactobacillus decrease, which may lead to the development of infection and severe complications during pregnancy. The aim of the study was the analysis of the bacterial composition of the vagina in 32 Caucasian women at each trimester of pregnancy using the next-generation sequencing method and primers targeting V3-V4 regions. In the studied group, the dominant species were Lactobacillus iners, Lactobacillus gasseri, and Lactobacillusplantarum. Statistically significant differences in the quantitative composition between trimesters were observed in relation to Lactobacillus jensenii,Streptococcus agalactiae, Lactobacillus iners, Gardnerella spp. Out of the 32 patients, 20 demonstrated fluctuations within the genus Lactobacillus, and 9 of them, at different stages of pregnancy, exhibited the presence of potentially pathogenic microbiota, among others: Streptococcus agalactiae, Gardnerella spp., Atopobium vaginae, and Enterococcus faecalis. The composition of the vaginal microbiota during pregnancy was subject to partial changes over trimesters. Although in one-third of the studied patients, both the qualitative and quantitative composition of microbiota was relatively constant, in the remaining patients, physiological and potentially pathogenic fluctuations were distinguished. |
| Databáze: | OpenAIRE |
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