Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy
| Autor: | Kiwamu Takahashi, Narumi Kunisue, Tohru Tanaka, Yoshiharu Okamoto, Masahiro Ishizuka, Tomohiro Osaki, Hideo Imazato, Urara Ota, Takuya Ishii |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
tumor Protoporphyrin IX Chemistry medicine.medical_treatment Neoplasms. Tumors. Oncology. Including cancer and carcinogens Photodynamic therapy Prodrug lung adenocarcinoma canine carcinoma eye diseases Article chemistry.chemical_compound Oncology photodynamic therapy Apoptosis Canine Lung Adenocarcinoma Cell culture medicine Cancer research polycyclic compounds Cytotoxicity therapeutics RC254-282 Intracellular |
| Zdroj: | Cancers Volume 13 Issue 16 Cancers, Vol 13, Iss 4174, p 4174 (2021) |
| ISSN: | 2072-6694 |
| Popis: | Photodynamic therapy (PDT) is a clinically approved, minimally invasive treatment for malignant tumors. Protoporphyrin IX (PpIX), derived from 5-aminolevulinic acid (5-ALA) as the prodrug, is one of the photosensitizers used in PDT. Recently, we reported a significant difference in response to 5-ALA-mediated PDT treatment in two canine primary lung adenocarcinoma cell lines (sensitive to PDT: HDC cells, resistant to PDT: LuBi cells). This study aimed to examine the difference in cytotoxicity of 5-ALA-mediated PDT in these cells. Although intracellular PpIX levels before irradiation were similar between HDC and LuBi cells, the percentage of ROS-positive cells and apoptotic cells in LuBi cells treated with 5-ALA-mediated PDT was significantly lower than that in HDC cells treated with 5-ALA-mediated PDT. A high dosage of the NO donor, DETA NONOate, significantly increased the cytotoxicity of 5-ALA-mediated PDT against LuBi cells. These results suggest that the sensitivity of 5-ALA-mediated PDT might be correlated with NO. |
| Databáze: | OpenAIRE |
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