Evaluation of an in vitro mast cell degranulation test in the context of food allergy to wheat
| Autor: | Claudine Guérin-Marchand, Marie Bodinier, Chantal Brossard, S. Triballeau, Sandra Denery-Papini, P. de Coppet, D.A. Moneret-Vautrin, F. Pineau, Ulrich Blank, Martine Morisset |
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| Přispěvatelé: | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie des Adaptations Nutritionnelles (PhAN), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Urticaria
Wheat Hypersensitivity WHEAT ALLERGY Immunoglobulin E FC EPSILON RI Cell Degranulation Gliadin TEST DE DEGRANULATION 0302 clinical medicine [SDV.IDA]Life Sciences [q-bio]/Food engineering HUMANIZED RAT CELL LINE Immunology and Allergy Fluorometry DERMATITE ATOMIQUE ANAPHYLAXIE A L'EFFORT 2. Zero hunger 0303 health sciences biology Reverse Transcriptase Polymerase Chain Reaction ALBUMIN/GLOBULIN FRACTION food and beverages General Medicine Middle Aged Mast cell Flow Cytometry medicine.anatomical_structure ELISA Adult Globulin Immunology Context (language use) Enzyme-Linked Immunosorbent Assay Transfection Cell Line Dermatitis Atopic 03 medical and health sciences OMEGA 5-GLIADINS Food allergy medicine Animals Humans [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering RNA Messenger IGE 030304 developmental biology Receptors IgE MAST CELLS Albumin Infant medicine.disease Rats RBL-2H3 biology.protein Wheat allergy 030215 immunology |
| Zdroj: | International Archives of Allergy and Immunology International Archives of Allergy and Immunology, Karger, 2008, 146 (4), pp.307-320. ⟨10.1159/000121465⟩ |
| ISSN: | 1018-2438 1423-0097 |
| DOI: | 10.1159/000121465⟩ |
| Popis: | Background: Antigenic profiles obtained by ELISA with IgE from patients with wheat food allergy (WFA) established that major allergens are albumins/globulins (AG) for children suffering from atopic eczema/dermatitis syndrome (AEDS), ω5-gliadins for adults suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA), anaphylaxis or urticaria and low-molecular-weight (LMW) glutenin subunits for patients with anaphylaxis. We aimed to characterize a new mast cell transfectant for its ability to degranulate with wheat proteins and patient sera and compare these results to those obtained by ELISA. Methods: Thirty sera from patients with WFA were tested: 14 with AEDS (group 1) and 16 with WDEIA, anaphylaxis or urticaria (group 2). An IgE Fc receptor (FcΕRI) humanized rat RBL-2H3 line was established by transfection with cDNAs encoding α-, β- and γ-subunits for the human IgE receptor. Results: A humanized RBL clone was selected for its capacity to express mRNA α-, β- and γ-subunits of FcΕRI, to bind allergen-specific human IgE and to degranulate. In group 1, sera induced enhanced degranulation with AG extract, but rarely reacted with gliadins and glutenins. In group 2, half of the sera showed degranulation with LMW glutenins whereas the AG fraction and lipid transfer proteins were rarely positive. ω5-Gliadins did not appear as a major allergen in degranulation assays, although functional allergen-specific IgE was measurable in appreciable amounts. Conclusion: Our data demonstrate that in wheat food allergen evaluation, correlation exists between mast cell degranulation and IgE measurements, depending on the type of allergen. Therefore, the biological activity of some allergen types may also be affected by other parameters. |
| Databáze: | OpenAIRE |
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