Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries
| Autor: | Mayumi Kajimura, C. C. Michel |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Potassium Channels Nitric Oxide Synthase Type III Physiology Indomethacin Rana temporaria Prostacyclin In Vitro Techniques Muscle Smooth Vascular Nitric oxide chemistry.chemical_compound Cyclic AMP medicine Animals Cyclooxygenase Inhibitors Splanchnic Circulation Rolipram Decerebrate State omega-N-Methylarginine Forskolin biology Rana pipiens Original Articles Epoprostenol Adenosine Capillaries Nitric oxide synthase Kinetics chemistry Biochemistry Permeability (electromagnetism) Biophysics biology.protein Nitric Oxide Synthase Intracellular medicine.drug |
| Zdroj: | The Journal of Physiology. 516:201-207 |
| ISSN: | 1469-7793 0022-3751 |
| DOI: | 10.1111/j.1469-7793.1999.201aa.x |
| Popis: | 1. We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (PK) of single perfused mesenteric microvessels in pithed frogs. 2. Neither superfusion with a nitric oxide synthase inhibitor, NG-monomethyl-L-arginine (10 or 100 micromol l-1), nor the addition of indomethacin (30 micromol l-1) to both perfusate and superfusate reduced the positive correlation between PK and flow velocity (U). 3. In the presence of agents known to raise intracellular levels of adenosine 3',5'-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between PK and U was no longer significant, so that PK was no longer flow dependent. 4. These results confirm that the flow dependence of PK is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of PK in frog mesenteric microvessels. |
| Databáze: | OpenAIRE |
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