Distribution of ethylene oxide in human blood and its implications for biomonitoring
| Autor: | H. M. Bolt, U. Föst, E. Hallier, H. Ottenwälder, H. Peter |
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| Rok vydání: | 1991 |
| Předmět: |
0301 basic medicine
Ethylene Oxide Health Toxicology and Mutagenesis Lymphocyte Toxicology 03 medical and health sciences chemistry.chemical_compound Plasma 0302 clinical medicine Blood plasma medicine Humans Carbon Radioisotopes Incubation chemistry.chemical_classification Blood Cells 030102 biochemistry & molecular biology Ethylene oxide General Medicine Glutathione In vitro Enzyme medicine.anatomical_structure chemistry Biochemistry Cytoplasm 030220 oncology & carcinogenesis Environmental Monitoring |
| Zdroj: | Humanexperimental toxicology. 10(1) |
| ISSN: | 0960-3271 |
| Popis: | The distribution of radioactivity following the incubation of human blood with radiolabelled ethylene oxide was investigated in vitro. After incubation, the individual blood samples were separated into lymphocytes and high (Mr > 10,000) and low (Mr < 10,000) molecular fractions of erythrocyte cytoplasm and blood plasma. The radioactivity was determined in each sample by liquid scintillation counting. In erythrocyte cytoplasm, the distribution of radioactivity showed marked interindividual differences and two distinct groups could be distinguished. The coincidence of these groups with 'conjugators' and 'non-conjugators', in terms of the enzymatic conjugation of methyl halides to glutathione in erythrocytes, suggests a common principle, such as enzyme polymorphism. Such polymorphism has been described for glutathione S-transferase μ in the human liver, an enzyme that efficiently conjugates epoxides. In the other blood compartments, the interindividual differences were either less significant or were not detectable. Binding products with various macromolecules in blood, such as haemoglobin or lymphocyte DNA, are being discussed as biological monitors for occupational exposure to ethylene oxide. The observation that erythrocytes exhibit interindividual differences as described above make binding products with haemoglobin less suitable for biological monitoring of ethylene oxide exposure than, for example, DNA adducts in lymphocytes. |
| Databáze: | OpenAIRE |
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