The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription
| Autor: | Ana I. Garrido-Godino, Abel Cuevas-Bermúdez, Francisco Navarro, Manuel Martín-Expósito, Antonio Fernández-Pevida, Verónica Martínez-Fernández, Sebastián Chávez, María Carmen Mirón-García, Jesús de la Cruz |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Genética |
| Rok vydání: | 2014 |
| Předmět: |
Genetics
Saccharomyces cerevisiae Proteins Transcription Elongation Genetic biology General transcription factor Gene regulation Chromatin and Epigenetics Nuclear Proteins Cell Cycle Proteins RNA polymerase II Non-coding RNA Chromatin Cell biology Peptide Initiation Factors Mutation biology.protein Transcriptional regulation Transcription factor II F RNA Polymerase II Transcription factor II D RNA polymerase II holoenzyme Small nuclear RNA |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname Nucleic Acids Research |
| Popis: | Bud27, the yeast orthologue of human URI/RMP, is a member of the prefoldin-like family of ATP-independent molecular chaperones. It has recently been shown to mediate the assembly of the three RNA polymerases in an Rpb5-dependent manner. In this work, we present evidence of Bud27 modulating RNA pol II transcription elongation. We show that Bud27 associates with RNA pol II phosphorylated forms (CTD-Ser5P and CTD-Ser2P), and that its absence affects RNA pol II occupancy of transcribed genes. We also reveal that Bud27 associates in vivo with the Sth1 component of the chromatin remodeling complex RSC and mediates its association with RNA pol II. Our data suggest that Bud27, in addition of contributing to Rpb5 folding within the RNA polymerases, also participates in the correct assembly of other chromatin-associated protein complexes, such as RSC, thereby modulating their activity. |
| Databáze: | OpenAIRE |
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