Waterborne microcystin-LR exposure induced chronic inflammatory response via MyD88-dependent toll-like receptor signaling pathway in male zebrafish
Autor: | Liping Yang, Lingkai Wang, Rong Tang, Li Li, Honghui Guo, Dandan Zhang, Xueyang Wu, Yuming Qiu, Wang Lin, Dapeng Li |
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Rok vydání: | 2020 |
Předmět: |
Male
Environmental Engineering Microcystins 010504 meteorology & atmospheric sciences Inflammation 010501 environmental sciences 01 natural sciences Immune system medicine Animals Environmental Chemistry Waste Management and Disposal Zebrafish 0105 earth and related environmental sciences Innate immune system biology Zebrafish Proteins biology.organism_classification Pollution Toll-like receptor signaling pathway Myeloid Differentiation Factor 88 Immunology Chronic inflammatory response Marine Toxins Tumor necrosis factor alpha Signal transduction medicine.symptom Water Pollutants Chemical |
Zdroj: | Science of The Total Environment. 702:134969 |
ISSN: | 0048-9697 |
Popis: | Waterborne microcystin-LR (MC-LR) released by cyanobacterial blooms in eutrophic water bodies have caused serious risk to aquatic animal and human health. In the present study, we for the first time conducted a comprehensive in vivo investigation on chronic inflammatory responses and its molecular pathways of different environmental relevant levels of MC-LR (0, 0.4, 2 and 10 μg/L) in male zebrafish (Danio rerio). The results showed that chronic MC-LR exposure caused splenic inflammatory changes including the formation of melano-macrophage centers, remarkable elevation of serum tumor necrosis factor alpha (TNFα) and interleukin 1 beta (IL1β) levels as well as significant upregulated expression of MyD88-dependent toll-like receptor (TLR/MyD88) signaling pathway genes (tlr4a, myd88, erk2, p38a, il1β and tnfα). The immunohistochemical and western blot results further validated that higher MC-LR concentrations tended to enhance the MyD88 signal. Moreover, significant decreases of serum C3 levels along with splenic c3b expression in the 10 μg/L exposure group proved that chronic MC-LR exposure could ultimately decrease the innate immunity of fish. Our findings revealed that chronic exposure of MC-LR could cause chronic inflammation through TLR/MyD88 signaling pathway and subsequently induce immune disorders in male zebrafish, which also urge us to pay more attention on the potential immunotoxicity of long-term exposure to low concentration of MC-LR. |
Databáze: | OpenAIRE |
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