Multi-protein Delivery by Nanodiamonds Promotes Bone Formation

Autor: Dean Ho, M. Gatica, H. Kim, E. Osawa, Laura K. Moore
Rok vydání: 2013
Předmět:
Surface Properties
Basic fibroblast growth factor
Cell Culture Techniques
Bone Morphogenetic Protein 2
Biocompatible Materials
Nanotechnology
02 engineering and technology
Bone morphogenetic protein
Bone morphogenetic protein 2
Nanodiamonds
Diffusion
Myoblasts
03 medical and health sciences
chemistry.chemical_compound
Drug Delivery Systems
Osteogenesis
Spectroscopy
Fourier Transform Infrared
medicine
Humans
Colloids
Particle Size
Progenitor cell
General Dentistry
Cell Proliferation
030304 developmental biology
0303 health sciences
Osteoblasts
Chemistry
Stem Cells
Cartilage
Cell Differentiation
Research Reports
Osteoblast
Alveolar Ridge Augmentation
Alkaline Phosphatase
021001 nanoscience & nanotechnology
Cell biology
medicine.anatomical_structure
Drug delivery
Fibroblast Growth Factor 2
0210 nano-technology
Protein Binding
Zdroj: Journal of Dental Research. 92:976-981
ISSN: 1544-0591
0022-0345
DOI: 10.1177/0022034513504952
Popis: Bone morphogenetic proteins (BMPs) are well-studied regulators of cartilage and bone development that have been Food and Drug Administration (FDA)-approved for the promotion of bone formation in certain procedures. BMPs are seeing more use in oral and maxillofacial surgeries because of recent FDA approval of InFUSE® for sinus augmentation and localized alveolar ridge augmentation. However, the utility of BMPs in medical and dental applications is limited by the delivery method. Currently, BMPs are delivered to the surgical site by the implantation of bulky collagen sponges. Here we evaluate the potential of detonation nanodiamonds (NDs) as a delivery vehicle for BMP-2 and basic fibroblast growth factor (bFGF). Nanodiamonds are biocompatible, 4- to 5-nm carbon nanoparticles that have previously been used to deliver a wide variety of molecules, including proteins and peptides. We find that both BMP-2 and bFGF are readily loaded onto NDs by physisorption, forming a stable colloidal solution, and are triggered to release in slightly acidic conditions. Simultaneous delivery of BMP-2 and bFGF by ND induces differentiation and proliferation in osteoblast progenitor cells. Overall, we find that NDs provide an effective injectable alternative for the delivery of BMP-2 and bFGF to promote bone formation.
Databáze: OpenAIRE
Externí odkaz: