OsSYL2 AA, an allele identified by gene‐based association, increases style length in rice (Oryza sativa L.)
| Autor: | Yanhui Li, Qiangming Liu, Dong She, Zhilan Fan, Shangshang Zhu, Xiaoxiao Hu, Delin Hong, Siqi Chen, Yulong Li, Bingjie Fang, Dalu Li, Yang Yang, Yufeng Wu, Erbao Liu, Xiaojing Dang, Yuanqing Zhang, Xiangong Chen, Hui Wang, Zexu Wu, Jie Ji |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine stigma characteristics Single-nucleotide polymorphism Outcrossing Genome-wide association study Plant Science Flowers Biology Genes Plant 01 natural sciences Polymorphism Single Nucleotide Linkage Disequilibrium 03 medical and health sciences Genetics natural variation Allele Gene Allele frequency Alleles genome‐wide association study Oryza sativa food and beverages Oryza Cell Biology Original Articles favorable alleles Hybrid seed 030104 developmental biology Genetics Population Original Article 010606 plant biology & botany hybrid rice seed production Genome-Wide Association Study |
| Zdroj: | The Plant Journal |
| ISSN: | 1365-313X 0960-7412 |
| Popis: | SUMMARY Stigma characteristics are important factors affecting the seed yield of hybrid rice per unit area. Natural variation of stigma characteristics has been reported in rice, but the genetic basis for this variation is largely unknown. We performed a genome‐wide association study on three stigma characteristics in six environments using 1.3 million single‐nucleotide polymorphism (SNPs) characterized in 353 diverse accessions of Oryza sativa. An abundance of phenotypic variation was present in the three stigma characteristics of these collections. We identified four significant SNPs associated with stigma length, 20 SNPs with style length (SYL), and 17 SNPs with the sum of stigma and style length, which were detected repeatedly in more than four environments. Of these SNPs, 28 were novel. We identified two causal gene loci for SYL, OsSYL3 and OsSYL2; OsSYL3 was co‐localized with the grain size gene GS3. The SYL of accessions carrying allele OsSYL3AA was significantly longer than that of those carrying allele OsSYL3CC. We also demonstrated that the outcrossing rate of female parents carrying allele OsSYL2AA increased by 5.71% compared with that of the isogenic line carrying allele OsSYL2CC in an F1 hybrid seed production field. The allele frequencies of OsSYL3AA and OsSYL2AA decreased gradually with an increase in latitude in the Northern Hemisphere. Our results should facilitate the improvement in stigma characteristics of parents of hybrid rice. Significance Statement The utilization of heterosis in rice is an effective strategy to enhance the grain yield. However, this entails the production of F1 hybrid seeds every year. The yield of F1 hybrid seeds is affected by stigma characteristics. The cloning of OsSYL2AA filled a gap in the genetic study of style length and could be used for improving stigma characteristics to increase the yield of F1 seeds. |
| Databáze: | OpenAIRE |
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