Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice
| Autor: | Tamara Saksida, Ivan Koprivica, Ivana Moric, Katarina Šavikin, Dragica Gajić, Lidija Senerovic, Ivana Stojanovic, Nada Pejnovic, Nebojša Menković |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
CD86 030109 nutrition & dietetics biology Chemistry Phagocytosis CD11c Spleen General Medicine medicine.disease_cause 3. Good health Microbiology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Listeria monocytogenes Integrin alpha M medicine biology.protein Cytotoxic T cell CD8 Food Science |
| Zdroj: | Food and Function |
| ISSN: | 2042-650X 2042-6496 |
| Popis: | Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiation in vitro. When applied orally to healthy mice, CE increased the proportion of CD11c+ dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingested Listeria monocytogenes as evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b+ macrophages and CD8+ cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86+ macrophages (CD11b+) and dendritic cells (CD11c+) was also enhanced in CE-pretreated infected mice. Further, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and the similar results were obtained in peritoneal macrophages in vitro. This effect of CE was associated with increased phosphorylation of IκB and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection with Listeria monocytogenes predominantly through increased activity of myeloid cells in the gut and in the spleen. This is the peer reviewed version of the following article: Gajić D, Saksida T, Koprivica I, Šenerović L, Morić I, Šavikin K, Menković N, Pejnović N, Stojanović I. Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice. Food Funct. 2020;[DOI:10.1039/d0fo00946f]. |
| Databáze: | OpenAIRE |
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