Longitudinal Studies on the Microcirculation around the TheraCyte™ Immunoisolation Device, Using the Laser Doppler Technique
Autor: | Gazelius B, Annika Tibell, G. S. Wu, E. Rafael |
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Rok vydání: | 2000 |
Předmět: |
Male
0301 basic medicine Microdialysis Cell Transplantation Swine Biomedical Engineering Neovascularization Physiologic lcsh:Medicine Capsules In Vitro Techniques Microcirculation Rats Sprague-Dawley Neovascularization 03 medical and health sciences 0302 clinical medicine In vivo Laser-Doppler Flowmetry medicine Animals Longitudinal Studies Transplantation business.industry Graft Survival lcsh:R Cell Biology Laser Doppler velocimetry Rats Microcirculatory flow 030104 developmental biology Regional Blood Flow medicine.symptom business Perfusion Blood Flow Velocity 030217 neurology & neurosurgery Biomedical engineering |
Zdroj: | Cell Transplantation, Vol 9 (2000) |
ISSN: | 1555-3892 0963-6897 |
DOI: | 10.1177/096368970000900113 |
Popis: | Encapsulation of cellular grafts in an immunoisolation membrane device may make it possible to perform transplantation without having to give immunosuppressive drugs. A common problem is the development of an avascular fibrotic zone around the implants, leading to impaired graft survival. The TheraCyte™ macroencapsulation device has therefore been designed to facilitate neovascularization of the device's surface. In this study, we evaluated the microcirculation around empty TheraCyte™ devices implanted SC in rats at various times after implantation, using a laser Doppler probe introduced via the device port. Studies were performed on day 1 or at 1, 2, and 4 weeks or at 2, 3, and 12 months after implantation. The mean flow was 158 ± 42, 148 ± 50, 133 ± 28, 72 ± 17, 138 ± 41, 165 ± 43, and 160 ± 29 perfusion units (PU), respectively. Thus, the microcirculation around the device was significantly reduced at 4 weeks after implantation (p < 0.01) while, from 2 months onwards the circulation had improved and did not differ significantly from that on day 1. The present study shows time-related changes in the microcirculatory flow around TheraCyte™ macroencapsulation devices that agree with our previous microdialysis studies on in vivo exchange of insulin and glucose between the device and the circulation. Laser Doppler flowmetry seems to provide a reliable technique for screening blood perfusion around macroencapsulation devices. |
Databáze: | OpenAIRE |
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