Supplementary Figure 2 from Dysregulation of the Repressive H3K27 Trimethylation Mark in Head and Neck Squamous Cell Carcinoma Contributes to Dysregulated Squamous Differentiation

Autor: Nicholas A. Saunders, Mehlika Hazar-Rethinam, Liliana Endo-Munoz, Lilia Merida de Long, Orla M. Gannon
Rok vydání: 2023
DOI: 10.1158/1078-0432.22454493.v1
Popis: PDF file - 1290K, Supplementary Figure 2. siRNA against EZH2 does not induce apoptosis. Detroit 562 cells and primary keratinocytes (HEK) were treated with siRNA against EZH2 for 48 hours or 96 hours and stained with Annexin V/7 aminoactinomycin D and subjected to FACS analysis to determine the proportion of apoptotic cells. a) The ability of siRNA to inhibit EZH2 was determined in the HEKs. β-actin is a loading control. b) EZH2 siRNA did not induce apoptosis after 48 hours or c) 96 hours. Data is mean � SEM, n=2
Databáze: OpenAIRE