Expression pattern and methylation of estrogen receptor α in breast intraductal proliferative lesions
| Autor: | Xinmiao Yu, Ayao Guo, Zhen Qiao, Chuifeng Fan, Xiaoyun Mao, Feng Jin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Cancer Research Pathology medicine.medical_specialty medicine.drug_class Estrogen receptor atypical ductal hyperplasia Breast Neoplasms Biology Polymerase Chain Reaction 03 medical and health sciences Young Adult 0302 clinical medicine Breast cancer ductal carcinoma in situ Carcinoma medicine Humans skin and connective tissue diseases Aged Aged 80 and over Hyperplasia Estrogen Receptor alpha Cancer estrogen receptor α General Medicine Methylation Articles usual ductal hyperplasia DNA Methylation Middle Aged medicine.disease Immunohistochemistry 030104 developmental biology Carcinoma Intraductal Noninfiltrating Oncology Estrogen 030220 oncology & carcinogenesis DNA methylation Female methylation Precancerous Conditions |
| Zdroj: | Oncology Reports |
| ISSN: | 1791-2431 1021-335X |
| Popis: | Intraductal proliferative lesions of the breast including usual ductal hyperplasia (UDH), atypical ductal hyperplasia (ADH) and ductal carcinoma in situ (DCIS) are associated with increased risk, albeit of greatly different magnitudes, for the subsequent development of invasive carcinoma. Estrogen receptor α (ERα) has been widely accepted as a prognostic marker and a predictor for endocrine therapy response of breast cancer. To investigate the ERα expression and methylation in breast intraductal proliferative lesions, we analyzed ERα expression in breast intraductal proliferative lesions including pure UDH (N=98), ADH without DCIS (N=160), DCIS without invasive breast cancer (N=149) by immunohistochemistry. Furthermore, the methylation status of ERα by methylation‑specific PCR (MSP) was defined in 217 cases of breast intraductal proliferative lesions. Immunohistochemistry showed that 98/98 (100%) of the UDH cases were positive for ERα expression. ERα protein expression in ADH (132/160) (92.5%) was higher than in DCIS (101/149) (67.8%). But the ERα expression pattern was different with histological diversity of breast intraductal proliferative lesions. The average percent cells staining positive for ERα was 35.33% in UDH, 87.75% in ADH and 71.45% in DCIS. ERα methylation in 32/60 (53.3%) UDH, 11/77 (10.2%) ADH and 32/80 (40.0%) DCIS. Our results demonstrated a strong negative correlation between the percent of cells staining positive for ERα and ERα methylation (r=‑0.831, p |
| Databáze: | OpenAIRE |
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