Nitric Oxide Overproduction by cue1 Mutants Differs on Developmental Stages and Growth Conditions
| Autor: | Oscar Lorenzo, Tamara Lechón, Luis Sanz, Inmaculada Sánchez-Vicente |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arginine nitric oxide homeostasis root development Mutant Endogeny Plant Science Carbohydrate metabolism 01 natural sciences stress responses 03 medical and health sciences chemistry.chemical_compound sugar metabolism Arabidopsis thaliana Nitric oxide homeostasis Ecology Evolution Behavior and Systematics Reactive nitrogen species Ecology biology Botany biology.organism_classification Cell biology cue1/nox1 030104 developmental biology reactive nitrogen species chemistry germination QK1-989 Phosphoenolpyruvate carboxykinase 010606 plant biology & botany |
| Zdroj: | Plants Volume 9 Issue 11 Plants, Vol 9, Iss 1484, p 1484 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9111484 |
| Popis: | The cue1 nitric oxide (NO) overproducer mutants are impaired in a plastid phosphoenolpyruvate/phosphate translocator, mainly expressed in Arabidopsis thaliana roots. cue1 mutants present an increased content of arginine, a precursor of NO in oxidative synthesis processes. However, the pathways of plant NO biosynthesis and signaling have not yet been fully characterized, and the role of CUE1 in these processes is not clear. Here, in an attempt to advance our knowledge regarding NO homeostasis, we performed a deep characterization of the NO production of four different cue1 alleles (cue1-1, cue1-5, cue1-6 and nox1) during seed germination, primary root elongation, and salt stress resistance. Furthermore, we analyzed the production of NO in different carbon sources to improve our understanding of the interplay between carbon metabolism and NO homeostasis. After in vivo NO imaging and spectrofluorometric quantification of the endogenous NO levels of cue1 mutants, we demonstrate that CUE1 does not directly contribute to the rapid NO synthesis during seed imbibition. Although cue1 mutants do not overproduce NO during germination and early plant development, they are able to accumulate NO after the seedling is completely established. Thus, CUE1 regulates NO homeostasis during post-germinative growth to modulate root development in response to carbon metabolism, as different sugars modify root elongation and meristem organization in cue1 mutants. Therefore, cue1 mutants are a useful tool to study the physiological effects of NO in post-germinative growth. |
| Databáze: | OpenAIRE |
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