A Molecular and Cellular Context-Dependent Role for Ir76b in Detection of Amino Acid Taste
| Autor: | Erika Varady, Anupama Dahanukar, Hanni Schoniger, Angelina Lee, Anindya Ganguly, Vi-Khoi Duong, Lisa Pang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Taste post-mating behavior Ionotropic Glutamate Medical Physiology Mutant feeding behavior Sodium Chloride Sodium Channels ionotropic receptors Animals Genetically Modified 0302 clinical medicine TAS1R3 Receptors Drosophila Proteins Amino Acids lcsh:QH301-705.5 chemistry.chemical_classification Neurons Microscopy Microscopy Confocal Behavior Animal GPR120 Amino acid TAS2R38 Drosophila melanogaster Phenotype Biochemistry Confocal Neurological Ir76b Female RNA Interference Drosophila 1.1 Normal biological development and functioning Genetically Modified Context (language use) Biology Receptors Ionotropic Glutamate General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Calcium imaging Underpinning research Genetics Animals Dental/Oral and Craniofacial Disease Nutrition Behavior Base Sequence Animal Neurosciences 030104 developmental biology chemistry lcsh:Biology (General) amino acid taste Calcium Biochemistry and Cell Biology CRISPR-Cas Systems Sugars 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 18, Iss 3, Pp 737-750 (2017) Cell reports, vol 18, iss 3 |
| ISSN: | 2211-1247 |
| Popis: | Amino acid taste is expected to be a universal property among animals. Although sweet, bitter, salt, and water tastes have been well characterized in insects, the mechanisms underlying amino acid taste remain elusive. From a Drosophila RNAi screen, we identifyan ionotropic receptor, Ir76b, as necessary for yeastpreference. Using calcium imaging, we identify Ir76b+ amino acid taste neurons in legs, overlapping partially with sweet neurons but not those that senseother tastants. Ir76b mutants have reduced responses to amino acids, which are rescued by transgenic expression of Ir76b and a mosquito ortholog AgIr76b. Co-expression of Ir20a with Ir76b is sufficient for conferring amino acid responses in sweet-taste neurons. Notably, Ir20a also serves to block salt response of Ir76b. Our study establishes the role of a highly conserved receptor in amino acid taste and suggests a mechanism for mutually exclusive roles of Ir76b in salt- and amino-acid-sensing neurons. |
| Databáze: | OpenAIRE |
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