Mechanism of poliovirus inactivation by bromine chloride
| Autor: | R S Fujioka, B H Keswick, Philip C. Loh |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Bromides
viruses chemistry.chemical_element medicine.disease_cause complex mixtures Applied Microbiology and Biotechnology Chloride Virus Viral Proteins Chlorides Centrifugation Density Gradient medicine Centrifugation Infectivity Bromine Ecology Chemistry Poliovirus RNA Biological activity Biochemistry RNA Viral Research Article Food Science Biotechnology medicine.drug |
| Zdroj: | Applied and Environmental Microbiology. 42:824-829 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | The mechanism of poliovirus inactivation by BrCl was determined by exposing poliovirus to various concentrations of BrCl and correlating the loss of virus infectivity with structural changes of the virus. Concentrations of 0.3 to 5 mg of BrCl per liter resulted in 95% to total inactivation of poliovirus. However, the inactivated virus retained structural integrity, as determined by buoyant density measurements of poliovirus labeled with radioactivity. However, at concentrations of 10 to 20 mg of BrCl per liter, total inactivation of poliovirus was associated with the degradation of the structural integrity of the virus. Since infectious ribonucleic acid at similar concentrations could be recovered from untreated poliovirus and poliovirus treated with 0.3 mg of BrCl per liter, it was concluded that BrCl as HOBr or bromamines inactivates poliovirus by reacting with the protein coat of the virus. Moreover, this inactivating reaction does not result in the degradation of the structure of the virion, nor does it affect the biological activity of the internal ribonucleic acid of the virus. |
| Databáze: | OpenAIRE |
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