Production of chimaeric bovine embryos and calves by aggregation of inner cell masses with morulae
| Autor: | I. Chartrain, W.A. King, L. Picard, K. J. Betteridge |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Freemartin Chromosomal translocation Immunosurgery Biology Morula Chromosomes Andrology Genetics Animals Inner cell mass Cell Aggregation Chimera Embryo Cell Biology Embryo Transfer Embryo Mammalian Embryo transfer Cell aggregation Microscopy Electron Blastocyst Phenotype Ultrastructure Cattle Female Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 27:295-304 |
| ISSN: | 1098-2795 1040-452X |
| Popis: | Bovine inner cell masses (ICMs) were isolated by immunosurgery at day 8 or 10, or by dissection at day 14, and combined with day-5.5 morulae. Aggregation was obtained between 89%, 62%, and 0% of the day-5:day-8, day-5:day-10, day-5:day-14 composites, respectively. Chromosome analysis of composites, respectively. Chromosome analysis of composites potentially carrying the 1/29 translocation as a chromosome marker and temporarily transferred to the bovine uterus for 8 days showed that chimaeric day-14 embryos can be obtained from day-5:day-8 aggregation. The definitive transfer of eight day-5:day-8 and 11 day-5:day-10 composites resulted in the birth of six and four calves, respectively; five of the six, but none of the four, were chimaeric. The five chimaeras showed mostly the ICM phenotype. The morphological differences between ICMs at different stages of development were examined by electron microscopy and related to the success of the aggregation technique. It is concluded that bovine embryonic cells can regulate for at least 3 days difference in development but not 5 days even though aggregation is still possible. |
| Databáze: | OpenAIRE |
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