cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata
| Autor: | Cui Sg, Haizhong Guo, Su Tf, Hu Yt, Dianchang Zhang, Shigui Jiang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Untranslated region
DNA Complementary Galectins Molecular Sequence Data Venerupis philippinarum Complementary DNA Genetics Animals Pinctada fucata Amino Acid Sequence Pinctada RNA Messenger Cloning Molecular Molecular Biology Phylogeny Galectin Gene Library Vibrio alginolyticus Expressed Sequence Tags Messenger RNA biology Base Sequence Gene Expression Profiling Galactosides General Medicine Sequence Analysis DNA biology.organism_classification Molecular biology Immunity Innate Open reading frame Gene Expression Regulation Tandem Repeat Sequences Sequence Alignment |
| Zdroj: | Genetics and molecular research : GMR. 10(3) |
| ISSN: | 1676-5680 |
| Popis: | Galectins can recognize and specifically bind to beta-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. We cloned the cDNA of a tandem-repeat galectin from the pearl oyster Pinctada fucata (designated as PoGal2). PoGal2 cDNA is 1347 bp long and consists of a 5'-untranslated region (UTR) of 3 bp, a 3'-UTR of 297 bp with one cytokine RNA instability motif (ATTTA), and an open reading frame of 1047 bp, encoding a polypeptide of 349 amino acids, with an estimated molecular mass of 38.1 kDa and a theoretical isoelectric point of 8.5. PoGal2 contains two carbohydrate recognition domains (CRDs); both have the conserved carbohydrate-binding motifs H-NPR and WG-EE. PoGal2 shares 50.6 and 50.9% identity with those of abalone (Haliotis discus) and the Manila clam (Venerupis philippinarum), respectively. Phylogenetic analysis revealed that the tandem-repeat galectins formed two clades for the different species. Molluscan tandem-repeat galectins were clustered into a single clade, and nematode tandem-repeat galectins were clustered into another single clade. In both clades, CRD-N and CRD-C were divided into different groups. PoGal2 mRNA was constitutively expressed in all tissues analyzed, and the expression level of PoGal2 mRNA was found to be significantly up-regulated in digestive glands, gills and hemocytes after Vibrio alginolyticus stimulation/infection. Expression profile analysis showed that the expression level of PoGal2 mRNA was significantly up-regulated at 8, 12 and 24 h after V. alginolyticus infection. These results suggest that PoGal2 is a constitutive and inducible acute-phase protein involved in the innate immune response of pearl oysters. |
| Databáze: | OpenAIRE |
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