A Novel Mechanism for Zika Virus Host-Cell Binding

Autor:	Courtney A. Rieder, Ryan F. Relich, James Vaughn, Sebastién Sannajust, Jonathan Rieder, Meghan May, Diana J. Goode, Tamara E. King, Derek C. Molliver, Ramaz Geguchadze
Rok vydání:	2019
Předmět:	0301 basic medicine ASN154 Amino Acid Motifs Cell Virus Attachment Phosphatidylserines Article Madin Darby Canine Kidney Cells Zika virus 03 medical and health sciences chemistry.chemical_compound Dogs 0302 clinical medicine Viral Envelope Proteins Annexin Virology Chlorocebus aethiops binding motif medicine Animals Humans 030212 general & internal medicine Asparagine Vero Cells Neurons Infectivity biology Zika Virus Infection Flavivirus Zika Virus Phosphatidylserine N-acetylglucosamine Fibroblasts biology.organism_classification 030104 developmental biology Infectious Diseases medicine.anatomical_structure chemistry Neurotropism Microcephaly Vero cell Encephalitis
Zdroj:	Viruses Volume 11 Issue 12
ISSN:	1999-4915
Popis:	Zika virus (ZIKV) recently emerged in the Western Hemisphere with previously unrecognized or unreported clinical presentations. Here, we identify two putative binding mechanisms of ancestral and emergent ZIKV strains featuring the envelope (E) protein residue asparagine 154 (ASN154) and viral phosphatidylserine (PS). Synthetic peptides representing the region containing ASN154 from strains PRVABC59 (Puerto Rico 2015) and MR_766 (Uganda 1947) were exposed to neuronal cells and fibroblasts to model ZIKV E protein/cell interactions and bound MDCK or Vero cells and primary neurons significantly. Peptides significantly inhibited Vero cell infectivity by ZIKV strains MR_766 and PRVABC59, indicating that this region represents a putative binding mechanism of ancestral African ZIKV strains and emergent Western Hemisphere strains. Pretreatment of ZIKV strains MR_766 and PRVABC59 with the PS-binding protein annexin V significantly inhibited replication of PRVABC59 but not MR_766, suggesting that Western hemisphere strains may additionally be capable of utilizing PS-mediated entry to infect host cells. These data indicate that the region surrounding E protein ASN154 is capable of binding fibroblasts and primary neuronal cells and that PS-mediated entry may be a secondary mechanism for infectivity utilized by Western Hemisphere strains.
Databáze:	OpenAIRE
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