Heterologous gene expression in Hansenula polymorpha: efficient secretion of glucoamylase
| Autor: | Peter Keup, Cornelis P. Hollenberg, Merckelbach Armin J, Alexander W.M. Strasser, Michael Piontek, Zbigniew A. Janowicz, Ulrike Weydemann, Gerd Gellissen |
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| Rok vydání: | 1991 |
| Předmět: |
Genes
Fungal Molecular Sequence Data Biomedical Engineering Bioengineering Biology Formate dehydrogenase Applied Microbiology and Biotechnology Pichia Plasmid Gene expression Amino Acid Sequence Cloning Molecular Gene chemistry.chemical_classification Promoter Molecular biology Yeast Enzyme Secretory protein chemistry Biochemistry Fermentation Saccharomycetales Molecular Medicine Glucan 1 4-alpha-Glucosidase Biotechnology Plasmids |
| Zdroj: | Bio/technology (Nature Publishing Company). 9(3) |
| ISSN: | 0733-222X |
| Popis: | We have introduced the glucoamylase gene (GAM1) from Schwanniomyces occidentalis into the genome of the methylo-trophic yeast Hansenula polymorpha to study the potential of this organism as a host for high-level expression of a heter-ologous gene encoding a secretory protein. Transformants of H. polymorpha containing GAM1 under control of the formate dehydrogenase (FMD) promoter are stable and efficiently secrete an active glucoamylase that is faithfully processed and modified. Yields of up to 1.4g/l of active enzyme were obtained at cell densities of 100–130 grams dry weight per liter. |
| Databáze: | OpenAIRE |
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