NMR Structure and IgE Epitopes of Blo t 21, a Major Dust Mite Allergen from Blomia tropicalis
| Autor: | Kang Ning Wong, Fook Tim Chew, Kang Wei Tan, Yunfeng Gao, Tan Ching Ong, Yu-Keung Mok, Yuen Sung Tiong |
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| Rok vydání: | 2012 |
| Předmět: |
Enzyme-Linked Immunosorbent Assay
Cross Reactions Biology medicine.disease_cause Immunoglobulin E Biochemistry Cross-reactivity Protein Structure Secondary Epitope Arthropod Proteins Epitopes Allergen immune system diseases otorhinolaryngologic diseases medicine Mite Animals Ige epitope Antigens Dermatophagoides Nuclear Magnetic Resonance Biomolecular Molecular Biology Blomia tropicalis Pyroglyphidae Cell Biology respiratory system Allergens biology.organism_classification Molecular biology Protein Structure Tertiary respiratory tract diseases Structural Homology Protein Protein Structure and Folding Immunology biology.protein |
| Zdroj: | Journal of Biological Chemistry. 287:34776-34785 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m112.348730 |
| Popis: | Blo t 21 is a paralogue of the group 5 allergen, Blo t 5, a major allergen from the dust mite Blomia tropicalis. Blo t 21 has moderate sequence identity (40.7%) to Blo t 5 and low to moderate cross-reactivity to Blo t 5. In B. tropicalis, the most prevalent and allergenic allergens are in the order of Blo t 21, Blo t 5, and Blo t 7. Here, we determined the NMR solution structure of Blo t 21, which represents the first structure of the group 21 dust mite allergen. The structure of Blo t 21 closely resembles the structures of Blo t 5 and Der p 5, comprising three anti-parallel α-helices arranged in a helical bundle. Using site-directed mutagenesis and specific IgE binding ELISA, Blo t 21 was found to contain both conserved and unique charged IgE epitope residues at the L2 loop region and on helix α3. Cross-inhibition assays confirmed that Blo t 21 has a low to moderate cross-reactivity with Blo t 5 and Der p 5 and represents a novel group of major allergen in B. tropicalis. In addition to group 5 allergens, Blo t 21 has also a low to moderate cross-reactivity with group 21 allergens from Dermatophagoides mites, confirming that B. tropicalis is a major and distinct source of dust mite allergens. |
| Databáze: | OpenAIRE |
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