DNase γ, DNase I and caspase-activated DNase cooperate to degrade dead cells
| Autor: | Daisuke Kitamura, Marie Kijima, Ryushin Mizuta, Makoto Yuasa, Shigetoshi Miura, Ryo Koyama, Shoko Sato, Tomoya Arai |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Apoptosis DNA Fragmentation 03 medical and health sciences Endonuclease chemistry.chemical_compound Caspase-activated DNase Mice Necrosis 0302 clinical medicine Multienzyme Complexes Cell Line Tumor Genetics Animals Humans Poly-ADP-Ribose Binding Proteins Mice Knockout Deoxyribonucleases Endodeoxyribonucleases biology Apoptotic DNA fragmentation DNA Degradation Necrotic Deoxyribonucleases Type I Site-Specific Cell Biology Molecular biology Chromatin 030104 developmental biology chemistry Liver 030220 oncology & carcinogenesis biology.protein DNA fragmentation DNase I hypersensitive site Female Hypersensitive site DNA Spleen |
| Zdroj: | Genes to cells : devoted to molecularcellular mechanisms. 21(11) |
| ISSN: | 1365-2443 |
| Popis: | Serum endonucleases are essential for degrading the chromatin released from dead cells and preventing autoimmune diseases such as systemic lupus erythematosus. Serum DNase I is known as the major endonuclease, but recently, another endonuclease, DNase γ/DNase I-like 3, gained attention. However, the precise role of each endonuclease, especially that of DNase γ, remains unclear. In this study, we distinguished the activities of DNase γ from those of DNase I in mouse serum and concluded that both cooperated in degrading DNA during necrosis: DNase γ functions as the primary chromatolytic activity, causing internucleosomal DNA fragmentation, and DNase I as the secondary one, causing random DNA digestion for its complete degradation. These results were confirmed by two in vivo experimental mouse models, in which necrosis was induced, acetaminophen-induced hepatic injury and streptozotocin-induced β-cell necrosis models. We also determined that DNase γ functions as a backup endonuclease for caspase-activated DNase (CAD) in the secondary necrosis phase after γ-ray-induced apoptosis in vivo. |
| Databáze: | OpenAIRE |
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