Role and origin of the GABAergic innervation of dorsal raphe serotonergic neurons

Autor: Patrice Fort, Guy Chouvet, Damien Gervasoni, Claire Rampon, Nadia Urbain, Bruno Barbagli, Pierre-Hervé Luppi, Christelle Peyron
Přispěvatelé: Physio-pathologie des réseaux neuronaux du cycle veille-sommeil, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en neurosciences de Lyon (CRNL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Team Physiopathologie des Réseaux Neuronaux Responsables du Cycle Veille-Sommeil, Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Neuropharmacologie et neurochimie, Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon
Předmět:
Male
Patch-Clamp Techniques
[SDV]Life Sciences [q-bio]
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MESH: Neurons
MESH: GABA Antagonists
GABA Antagonists
Rats
Sprague-Dawley
0302 clinical medicine
MESH: Animals
gamma-Aminobutyric Acid
Neurons
0303 health sciences
MESH: Electrophysiology
Glutamate Decarboxylase
General Neuroscience
Serotonergic cell groups
MESH: Raphe Nuclei
Electroencephalography
Iontophoresis
Immunohistochemistry
Electrophysiology
Preoptic area
GABAergic
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
MESH: Iontophoresis
MESH: Glutamate Decarboxylase
Cholera Toxin
Serotonin
MESH: Rats
Sleep
REM
Biology
Bicuculline
Serotonergic
Periaqueductal gray
MESH: Electromyography
03 medical and health sciences
Dorsal raphe nucleus
MESH: Bicuculline
MESH: Rats
Sprague-Dawl
MESH: Patch-Clamp Techniques
MESH: Electroencephalography
Animals
ARTICLE
MESH: Cholera Toxin
030304 developmental biology
Electromyography
MESH: Immunohistochemistry
Receptors
GABA-A
Retrograde tracing
MESH: Male
Rats
Raphe Nuclei
Sleep
Neuroscience
030217 neurology & neurosurgery
Zdroj: ResearcherID
Journal of Neuroscience
Journal of Neuroscience, Society for Neuroscience, 2000, 20 (11), pp.4217-25
Journal of Neuroscience, Society for Neuroscience, 2000, 20 (11), pp.4217-25. ⟨10.1523/JNEUROSCI.20-11-04217.2000⟩
Europe PubMed Central
Scopus-Elsevier
ISSN: 0270-6474
1529-2401
DOI: 10.1523/JNEUROSCI.20-11-04217.2000⟩
Popis: Extracellular electrophysiological recordings in freely moving cats have shown that serotonergic neurons from the dorsal raphe nucleus (DRN) fire tonically during wakefulness, decrease their activity during slow wave sleep (SWS), and are nearly quiescent during paradoxical sleep (PS). The mechanisms at the origin of the modulation of activity of these neurons are still unknown. Here, we show in the unanesthetized rat that the iontophoretic application of the GABAAantagonist bicuculline on dorsal raphe serotonergic neurons induces a tonic discharge during SWS and PS and an increase of discharge rate during quiet waking. These data strongly suggest that an increase of a GABAergic inhibitory tone present during wakefulness is responsible for the decrease of activity of the dorsal raphe serotonergic cells during slow wave and paradoxical sleep. In addition, by combining retrograde tracing with cholera toxin B subunit and glutamic acid decarboxylase immunohistochemistry, we demonstrate that the GABAergic innervation of the dorsal raphe nucleus arises from multiple distant sources and not only from interneurons as classically accepted. Among these afferents, GABAergic neurons located in the lateral preoptic area and the pontine ventral periaqueductal gray including the DRN itself could be responsible for the reduction of activity of the serotonergic neurons of the dorsal raphe nucleus during slow wave and paradoxical sleep, respectively.
Databáze: OpenAIRE
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