The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways
| Autor: | Alfons J. M. Stams, Steffen Kümmel, Steffi Franke, Sjef Boeren, Antonie H. van Gelder, Diana Z. Sousa, Peter D. E. M. Verhaert, Martijn W. H. Pinkse, Carsten Vogt, Michael Visser, Mervin Pieterse |
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| Přispěvatelé: | Imaging Mass Spectrometry (IMS), RS: M4I - Imaging Mass Spectrometry (IMS), Universidade do Minho |
| Rok vydání: | 2018 |
| Předmět: |
Proteomics
0301 basic medicine Microorganism MOORELLA-THERMOACETICA ved/biology.organism_classification_rank.species Gene Expression General Physics and Astronomy Biochemistry chemistry.chemical_compound Microbiologie lcsh:Science Phylogeny Multidisciplinary biology Chemistry Hydrolysis METHANOSARCINA-BARKERI Cobalt SPOROMUSA-OVATA HYDROGEN ENCODING GENES 6. Clean water Vitamin B 12 Desulfotomaculum BACTERIUM Oxidation-Reduction Metabolic Networks and Pathways STABLE-ISOTOPE FRACTIONATION Science 030106 microbiology M METHYLTRANSFERASE Biochemie Microbiology Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Bacterial Proteins Moorella thermoacetica ANAEROBIC DEGRADATION Life Science Sulfate Science & Technology WIMEK Methanol dehydrogenase ved/biology Gene Expression Profiling Methanol Thermophile Alcohol Dehydrogenase Methyltransferases General Chemistry biology.organism_classification Culture Media 030104 developmental biology SP-NOV lcsh:Q Methanosarcina barkeri Bacteria |
| Zdroj: | Nature Communications, 9(1) Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018) Nature Communications, 9:239. Nature Publishing Group Nature Communications 9 (2018) 1 Nature Communications Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-017-02518-9 |
| Popis: | Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment. Research was funded by grants of the Division of Chemical Sciences (CW-TOP 700.55.343) and Earth and Life Sciences (ALW 819.02.014) of The Netherlands Organisation for Scientific Research (NWO), the European Research Council (ERC grant 323009), and the Gravitation grant (024.002.002) of the Netherlands Ministry of Education, Culture and Science info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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