Expression and association of carbonic anhydrase IX and cyclooxygenase-2 in colorectal cancer
| Autor: | Veronika Tupá, Marian Adamkov, Slávka Drahošová, Marian Grendar |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Colorectal cancer Inflammation Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine medicine Biomarkers Tumor Humans Neoplasm Invasiveness Carbonic Anhydrase IX Aged chemistry.chemical_classification Aged 80 and over biology Chemistry Cell Biology Hypoxia (medical) Middle Aged medicine.disease Prognosis Molecular biology Immunohistochemistry 030104 developmental biology Enzyme Cytoplasm Cyclooxygenase 2 030220 oncology & carcinogenesis biology.protein Female Cyclooxygenase medicine.symptom Colorectal Neoplasms |
| Zdroj: | Pathology, research and practice. 215(4) |
| ISSN: | 1618-0631 |
| Popis: | Background This work was designed to determine the relationship between hypoxia-inducible protein carbonic anhydrase IX (CA IX) and pro-inflammatory enzyme cyclooxygenase-2 (COX-2) in patients with colorectal cancer (CRC). Methods We examined CA IX and COX-2 expression in CRC tissues by immunohistochemical staining of 111 samples. We evaluated the correlation between the expression of these proteins and their correlation with the clinico-morphological parameters of CRC. Results CA IX was detected in 89 of 111 cases (80.2%). We predominantly observed membrane staining (70.3%) and a strong immunoreaction intensity (58.6%). The percentage of labeled cells in malignant lesions was less than 25% in 12.6% of cases, less than 50% in 15.3% of cases and more than 50% in 52.3% of CRC cases. The COX-2 protein was expressed in 94 of 111 cases (84.7%). We noticed only cytoplasmic localization, while immunoreaction intensity was predominantly strong (47.8%). The percentage of COX-2 positive cells was less than 25% only in 2.7% of the cases, less than 50% in 21.6% of the cases and more than 50% in 60.4% of the cases. No statistically significant correlations were observed between CA IX expression and clinico-morphological parameters. COX-2 expression was only significantly correlated with the tumor stage. Statistical analysis confirmed a significant correlation between the parameters of expression of the CA IX and COX-2 proteins. Conclusion CA IX/COX-2 interplay can promote hypoxia survival and the invasion of tumor cells. These proteins may represent independent prognostic factors of CRC. |
| Databáze: | OpenAIRE |
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