Isolation of Epstein-Barr-Virus-Transformed Lymphocytes Producing IgG Class Monoclonal Antibodies Using a Magnetic Cell Separator (MACS): Preparation of Thyroid-Stimulating IgG Antibodies from Patients with Graves′ Disease
| Autor: | Fumihiko Matsuda, Jyoji Okuda, Hua Li, Toru Mori, Hideo Sugawa, Takeshi Tsubata, Takashi Akamizu |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Herpesvirus 4
Human endocrine system endocrine system diseases medicine.drug_class Graves' disease Biophysics Biotin medicine.disease_cause Monoclonal antibody Biochemistry Virus Antibody Specificity medicine Humans Lymphocytes Molecular Biology B cell Cell Line Transformed biology Immunomagnetic Separation Autoantibody Antibodies Monoclonal Cell Biology medicine.disease Epstein–Barr virus Virology Graves Disease Clone Cells Immunoglobulin Isotypes medicine.anatomical_structure Immunoglobulin M Immunoglobulin G Immunology Monoclonal biology.protein Antibody Immunoglobulins Thyroid-Stimulating |
| Zdroj: | Biochemical and Biophysical Research Communications. 207:985-993 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1995.1282 |
| Popis: | In autoimmune diseases, IgG class autoantibodies are generally considered to be more pathognomonic than IgM class ones. Although Epstein-Barr virus (EBV)-transformation of lymphocytes is a useful method to obtain human monoclonal autoantibodies, it tends to result predominantly in IgM-producing cells. We depleted IgM + cells before EBV-transformation with a Magnetic Cell Separator (MACS) in order to increase the chance of acquisition of cells producing IgG class anti-thyrotropin (TSH) receptor antibodies (TRAb). As a result, we obtained four independent B cell clones producing IgG class monoclonal thyroid-stimulating antibodies (TSAb) from three patients with Graves′ disease. None of these clones showed any TSH binding inhibitor immunoglobulin (TBII) activity, suggesting independence of TSAb-producing lymphocytes from those producing TBII. |
| Databáze: | OpenAIRE |
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