Nicorandil decreases oxidative stress in slow‐ and fast‐twitch muscle fibers of diabetic rats by improving the glutathione system functioning
| Autor: | Sergio Márquez-Gamiño, Elizabeth Sánchez-Duarte, Sarai Sánchez-Duarte, Fernando Sotelo-Barroso, Cipriana Caudillo-Cisneros, Erick A. Villicaña-Gómez, Ma. Teresa Melchor-Moreno, Rocío Montoya-Pérez, Karla S. Vera-Delgado |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Basic Science and Research medicine.medical_specialty Transcription Genetic Endocrinology Diabetes and Metabolism Diabetic myopathy 030204 cardiovascular system & hematology medicine.disease_cause Streptozocin Diseases of the endocrine glands. Clinical endocrinology Diabetes Mellitus Experimental Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Internal Medicine medicine TBARS Animals Rats Wistar Muscle Skeletal Nicorandil Soleus muscle business.industry Insulin tolerance test Articles General Medicine Glutathione Streptozotocin RC648-665 Rats Muscle Fibers Slow-Twitch 030104 developmental biology Endocrinology Gene Expression Regulation chemistry Oxidative stress Muscle Fibers Fast-Twitch Original Article Lipid Peroxidation business Oxidation-Reduction medicine.drug |
| Zdroj: | Journal of Diabetes Investigation, Vol 12, Iss 7, Pp 1152-1161 (2021) Journal of Diabetes Investigation |
| ISSN: | 2040-1116 2040-1124 |
| Popis: | Aims/Introduction Myopathy is a common complication of any diabetes type, consisting in failure to preserve mass and muscular function. Oxidative stress has been considered one of the main causes for this condition. This study aimed to search if Nicorandil, a KATP channel opener, could protect slow‐ and fast‐twitch diabetic rat muscles from oxidative stress, and to unveil its possible mechanisms. Materials and Methods Diabetes was induced in male Wistar rats by applying intraperitoneally streptozotocin (STZ) at 100 mg/kg doses. Nicorandil (3 mg/kg/day) was administered along 4 weeks. An insulin tolerance test and assessment of fasting blood glucose (FBG), TBARS, reduced (GSH), and disulfide (GSSG) glutathione levels, GSH/GSSG ratio, and mRNA expression of glutathione metabolism‐related genes were performed at end of treatment in soleus and gastrocnemius muscles. Results Nicorandil significantly reduced FBG levels and enhanced insulin tolerance in diabetic rats. In gastrocnemius and soleus muscles, Nicorandil attenuated the oxidative stress by decreasing lipid peroxidation (TBARS), increasing total glutathione and modulating GPX1‐mRNA expression in both muscle’s types. Nicorandil also increased GSH and GSH/GSSG ratio and downregulated the GCLC‐ and GSR‐mRNA in gastrocnemius, without significative effect on those enzymes’ mRNA expression in diabetic soleus muscle. Conclusions In diabetic rats, Nicorandil attenuates oxidative stress in slow‐ and fast‐twitch skeletal muscles by improving the glutathione system functioning. The underlying mechanisms for the modulation of glutathione redox state and the transcriptional expression of glutathione metabolism‐related genes seem to be fiber type‐dependent. |
| Databáze: | OpenAIRE |
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