Emission of ent-kaurene, a diterpenoid hydrocarbon precursor for gibberellins, into the headspace from plants
Autor: | Tomonobu Toyomasu, Wataru Mitsuhashi, Kazunori Okada, Hiromichi Kenmoku, Takeshi Sassa, Shinjiro Yamaguchi, Yuji Kamiya, Minoru Otsuka, Mikihiro Ogawa |
---|---|
Rok vydání: | 2004 |
Předmět: |
Physiology
Mutant Plant Science Biology chemistry.chemical_compound Biosynthesis Arabidopsis otorhinolaryngologic diseases Arabidopsis thaliana DNA Primers Alkyl and Aryl Transferases ATP synthase Base Sequence Reverse Transcriptase Polymerase Chain Reaction fungi Wild type food and beverages Cell Biology General Medicine biology.organism_classification Plants Genetically Modified Gibberellins Biochemistry chemistry biology.protein Gibberella fujikuroi Gibberellin Diterpenes Kaurane |
Zdroj: | Plantcell physiology. 45(9) |
ISSN: | 0032-0781 |
Popis: | ent-Kaurene is a tetracyclic hydrocarbon precursor for gibberellins (GAs) in plants and fungi. To address whether fungal GA biosynthesis enzymes function in plants, we generated transgenic Arabidopsis plants overexpressing ent-kaurene synthase (GfCPS/KS) from a GA-producing fungus Gibberella fujikuroi. GfCPS/KS catalyzes a two-step reaction corresponding to ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) activities in plants. When GfCPS/KS was overexpressed and targeted to plastids, a range of GA-deficient phenotypes of the ga1-3 and ga2-1 mutants (defective in CPS and KS, respectively) were restored to wild type. Unexpectedly, the transgenic lines overproducing GfCPS/KS emitted the GA precursor ent-kaurene into the headspace besides its accumulation in the plant body. When co-cultivated with the ent-kaurene overproducers in a closed environment, the airborne ent-kaurene was able to fully complement the dwarf phenotype of ga1-3 and ga2-1 mutants, but not that of the ga3-1 mutant (defective in ent-kaurene oxidase). These results suggest that ent-kaurene may be efficiently metabolized into bioactive GAs in Arabidopsis when supplied as a volatile. We also provide evidence that ent-kaurene is released in the headspace of wild-type Chamaecyparis obtusa and Cryptomeria japonica plants, suggesting the occurrence of this hydrocarbon GA precursor as a volatile in nature. |
Databáze: | OpenAIRE |
Externí odkaz: |