Case study of the morphologic variation of circulating tumor cells
| Autor: | Nicole Hanson Lazarus, Kelly Bethel, Lindsay Kroener, Robert T. Krivacic, Ben Hsieh, Joan Kroener, Peter Kuhn, Douglas N. Curry, Andras Ladanyi, Jorge Nieva, Dena Marrinucci, Richard H. Bruce, Mark Humphrey |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Bone Neoplasms Breast Neoplasms Pathology and Forensic Medicine Metastasis Fatal Outcome Breast cancer Circulating tumor cell Biopsy medicine Carcinoma Fiber Optic Technology Humans Optical Fibers medicine.diagnostic_test business.industry Carcinoma Ductal Breast Neoplastic Cells Circulating medicine.disease medicine.anatomical_structure Pleomorphism (cytology) Female Cytophotometry Bone marrow business Breast carcinoma |
| Zdroj: | Human Pathology. 38:514-519 |
| ISSN: | 0046-8177 |
| Popis: | We report a detailed cytomorphologic evaluation of the circulating component of widely metastatic breast carcinoma. A previously healthy 38-year-old woman was diagnosed with breast cancer. Wide local excision revealed a 1.7-cm infiltrating ductal adenocarcinoma, BSR score 7/9 with angiolymphatic invasion, and 4/20 lymph nodes positive for carcinoma. Five years later, a bone marrow biopsy revealed involvement of bone marrow by metastatic breast carcinoma, and shortly thereafter, metastases were identified in the liver and lung hilum. She enrolled in a clinical investigation for the detection of circulating tumor cells (CTCs) in breast carcinoma. A total of 659 CTCs were identified in a 10-mL blood sample using an immunofluorescent protocol targeting cytokeratins and detected using fiber-optic array scanning technology. The detected CTCs were subsequently stained with a Wright-Giemsa stain, and representative cells were evaluated in detail by light microscopy for morphologic evaluation. We find that the patient's CTCs exhibit a high degree of pleomorphism including CTCs with high and low nuclear-to-cytoplasmic ratios along with CTCs exhibiting early and late apoptotic changes. In addition, in comparison with her tumor cells in other sites, the full morphologic spectrum of cancer cells present in primary and metastatic tumor is also present in peripheral blood circulation. |
| Databáze: | OpenAIRE |
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