Marine biomaterials: Biomimetic and pharmacological potential of cultivated Aplysina aerophoba marine demosponge
| Autor: | Björn Binnewerg, Mario Schubert, Kaomei Guan, Marcin Wysokowski, Antje Tunger, Marco Giovine, Stefan R. Bornstein, Grigory V. Zyryanov, Marc Schmitz, Allison L. Stelling, Andriy Fursov, L. V. Muzychka, Mirko Djurovic, Iaroslav Petrenko, Viatcheslav N. Ivanenko, Olga S. Taniya, Nicole Bechmann, Oleg B. Smolii, Hermann Ehrlich, Alona Voronkina, Igor S. Kovalev, Rajko Martinović, Yvonne Joseph, Konstantin R. Tabachnick, Valentine Kovalchuk, Mikhail V. Tsurkan |
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| Přispěvatelé: | University of Zurich, Wysokowski, Marcin |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Aquatic Organisms
10265 Clinic for Endocrinology and Diabetology 2210 Mechanical Engineering Chitin 02 engineering and technology 01 natural sciences Extracellular matrix chemistry.chemical_compound Tissue engineering Biomimetic Materials Myocytes Cardiac Cardiomyocytes biology Biomaterial 021001 nanoscience & nanotechnology Aeroplysinin-1 Porifera Biochemistry Mechanics of Materials MCF-7 Cells 0210 nano-technology medicine.drug Acetonitriles Materials science 3104 Condensed Matter Physics Biocompatibility Induced Pluripotent Stem Cells Bioengineering 610 Medicine & health Secondary metabolite 010402 general chemistry Biomaterials Alkaloids 2211 Mechanics of Materials Cell Line Tumor Cyclohexenes medicine Animals Humans biology.organism_classification 2500 General Materials Science 0104 chemical sciences Cell culture Marine biomaterials Sponge chemistry Delayed-Action Preparations |
| Popis: | Marine demosponges of the Verongiida order are considered a gold-mine for bioinspired materials science and marine pharmacology. The aim of this work was to simultaneously isolate selected bromotyrosines and unique chitinous structures from A. aerophoba and to propose these molecules and biomaterials for possible application as antibacterial and antitumor compounds and as ready-to-use scaffolds for cultivation of cardiomyocytes, respectively. Among the extracted bromotyrosines, the attention has been focused on aeroplysinin-1 that showed interesting unexpected growth inhibition properties for some Gram-negative clinical multi-resistant bacterial strains, such as A. baumannii and K. pneumoniae, and on aeroplysinin-1 and on isofistularin-3 for their anti-tumorigenic activity. For both compounds, the effects are cell line dependent, with significant growth inhibition activity on the neuroblastoma cell line SH-SY5Y by aeroplysinin-1 and on breast cancer cell line MCF-7 by isofistularin-3. In this study, we also compared the cultivation of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) on the A. aerophoba chitinous scaffolds, in comparison to chitin structures that were pre-coated with Geltrex™, an extracellular matrix mimetic which is used to enhance iPSC-CM adhesion. The iPSC-CMs on uncoated and pure chitin structures started contracting 24 h after seeding, with comparable behaviour observed on Geltrex-coated cell culture plates, confirming the biocompatibility of the sponge biomaterial with this cell type. The advantage of A. aerophoba is that this source organism does not need to be collected in large quantities to supply the necessary amount for further pre-clinical studies before chemical synthesis of the active compounds will be available. A preliminary analysis of marine sponge bioeconomy as a perspective direction for application of biomaterials and secondary bioactive metabolites has been finally performed for the first time. |
| Databáze: | OpenAIRE |
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