Intestinal CD36. A lipid-sensor involved in the processing of chylomicrons in rodents
| Autor: | L. Clement, H. Poirier, Jan F. C. Glatz, Fatiha Nassir, I. Niot, V. Petit, Nada A. Abumrad, Maurice M.A.L. Pelsers, Thi Tran, M.-C. Monnot, Pascal Degrace, Philippe Besnard |
|---|---|
| Přispěvatelé: | Physiologie de la Nutrition et Toxicologie (NUTox) (U866, Lipides et nutrition, équipe 7) (NUTox), Lipides - Nutrition - Cancer (U866) (LNC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Washington University in Saint Louis (WUSTL), Maastricht University [Maastricht], Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
030309 nutrition & dietetics
CD36 [SDV]Life Sciences [q-bio] 030209 endocrinology & metabolism 03 medical and health sciences Oleoylethanolamide chemistry.chemical_compound 0302 clinical medicine In vivo parasitic diseases medicine Lipid sensor General Psychology 0303 health sciences Nutrition and Dietetics biology Chinese hamster ovary cell Small intestine 3. Good health Cell biology medicine.anatomical_structure chemistry Proteasome inhibitor biology.protein lipids (amino acids peptides and proteins) [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Ex vivo medicine.drug Chylomicron |
| Zdroj: | Appetite Appetite, Elsevier, 2011, 57 (Supplement 1), pp.S44. ⟨10.1016/j.appet.2011.05.284⟩ |
| ISSN: | 0195-6663 1095-8304 |
| DOI: | 10.1016/j.appet.2011.05.284⟩ |
| Popis: | International audience; CD36 is a multifunctional glycoprotein which binds nanomolar concentrations of long-chain fatty acids (LCFA) and is highly expressed on the luminal surface of enterocytes. Despite of its implication in oleoylethanolamide (OEA) and chylomicron synthesis, CD36 function in small intestine remains incompletely understood. Our in vivo data demonstrated that CD36 gene deletion in mice did not affect intestinal LCFA uptake. CD36 protein disappeared early from the luminal side of intestinal villi during the post-prandial period but only when the diet contained lipids. This drop was significant 1 h after a lipid supply and was associated with ubiquitination of CD36. Using CHO cells expressing CD36, it was shown that the digestion products, LCFA and diglycerides, trigger CD36 ubiquitination. In vivo treatment with a proteasome inhibitor prevented the lipid-mediated degradation of CD36. In vivo and ex vivo, CD36 was required for dietary-lipid activation of ERK1/2 which is associated ex vivo with an increase of ApoB48 and MTP, proteins involved in chylomicron formation. Therefore, intestinal CD36 through ERK1/2 mediated signaling displays features of a lipid sensor involved in the adaptation of intestinal metabolism to the postprandial lipid challenge by promoting the production of large chylomicrons rapidly cleared in the blood. Supported by: National Institute of Health and Medical Research (INSERM) and the National Institute of Agronomic Research (INRA) and by grants from the Research Program in Human Nutrition SensoFAT, French National. |
| Databáze: | OpenAIRE |
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